Antibacterial Activity of Alkyl Gallates against Xanthomonas citri subsp citri

Autor(es)

Silva, I. C. ^[1] ; Regasini, L. O. ^[2] ; Petronio, M. S. ^[2] ; Silva, D. H. S. ^[2] ; Bolzani, V. S. ^[2] ; Belasque, Jr., J. ^[3] ; Sacramento, L. V. S. ^[4] ; Ferreira, H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Ciencias Biol, FCF, Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Inst Quim, Dept Quim Organ, Sao Paulo - Brazil

^[3] Fundecitrus, Dept Cient, Sao Paulo - Brazil

^[4] Univ Estadual Paulista, FCF, Dept Principios Ativos Nat & Toxicol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Bacteriology; v. 195, n. 1, p. 85-94, JAN 2013.
• Citações Web of Science: 25

Resumo

The plant-pathogenic bacterium Xanthomonas citri subsp. citri is the causal agent of Asiatic citrus canker, a serious disease that affects all the cultivars of citrus in subtropical citrus-producing areas worldwide. There is no curative treatment for citrus canker; thus, the eradication of infected plants constitutes the only effective control of the spread of X. citri subsp. citri. Since the eradication program in the state of Sao Paulo, Brazil, is under threat, there is a clear risk of X. citri subsp. citri becoming endemic in the main orange-producing area in the world. Here we evaluated the potential use of alkyl gallates to prevent X. citri subsp. citri growth. These esters displayed a potent anti-X. citri subsp. citri activity similar to that of kanamycin (positive control), as evaluated by the resazurin microtiter assay (REMA). The treatment of X. citri subsp. citri cells with these compounds induced altered cell morphology, and investigations of the possible intracellular targets using X. citri subsp. citri strains labeled for the septum and centromere pointed to a common target involved in chromosome segregation and cell division. Finally, the artificial inoculation of citrus with X. citri subsp. citri cells pretreated with alkyl gallates showed that the bacterium loses the ability to colonize its host, which indicates the potential of these esters to protect citrus plants against X. citri subsp. citri infection. (AU)

• Processo FAPESP: 10/02667-4 - Prospecção de compostos naturais com potencial para inibir divisão celular em Xanthomonas citri subsp. citri
• Beneficiário: Isabel Cristiane da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/09173-6 - Caracterização funcional de proteínas hipotéticas de bactérias fitopatogênicas
• Beneficiário: Henrique Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores