Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of the taxonomic status of populations assigned to Phyllomedusa hypochondrialis (Anura, Hylidae, Phyllomedusinae) based on molecular, chromosomal, and morphological approach

Texto completo
Autor(es):
Bruschi, Daniel Pacheco [1] ; Busin, Carmen Silvia [2] ; Toledo, Luis Felipe [3] ; Vasconcellos, Gilda Andrade [4] ; Strussmann, Christine [5] ; Weber, Luiz Norberto [4] ; Lima, Albertina Pimentel [6] ; Lima, Jucivaldo Dias [7] ; Recco-Pimentel, Shirlei Maria [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Estrutural & Func, BR-13083863 Campinas, SP - Brazil
[2] Univ Passo Fundo, Inst Ciencias Biol, Lab Citogenet, BR-99001970 Passo Fundo, RS - Brazil
[3] Univ Estadual Campinas, Inst Biol, Museu Zool Prof Adao Jose Cardoso, BR-13083863 Campinas, SP - Brazil
[4] Univ Fed Maranhao, Ctr Ciencias Saude, Dept Biol, BR-65085580 Sao Luis, MA - Brazil
[5] Univ Fed Mato Grosso, Fac Agron Med Vet & Zootecnia, Dept Ciencias Basicas & Prod Anim, BR-78060900 Cuiaba, MT - Brazil
[6] Inst Nacl de Pesquisas da Amazonia, BR-69060001 Manaus, Amazonas - Brazil
[7] Inst Pesquisas Cient & Tecnol Estado Amapa, Div Zool, BR-68912250 Macapa, AP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: BMC GENETICS; v. 14, AUG 12 2013.
Citações Web of Science: 15
Resumo

Background: The taxonomic and phylogenetic relationships of the genus Phyllomedusa have been amply discussed. The marked morphological similarities among some species hamper the reliable identification of specimens and may often lead to their incorrect taxonomic classification on the sole basis of morphological traits. Phenotypic variation was observed among populations assigned to either P. azurea or P. hypochondrialis. In order to evaluate whether the variation observed in populations assigned to P. hypochondrialis is related to that in genotypes, a cytogenetic analysis was combined with phylogenetic inferences based on mitochondrial and nuclear sequences. Results: The inter-and intra-population variation in the external morphology observed among the specimens analyzed in the present study do not reflect the phylogenetic relationships among populations. A monophyletic clade was recovered, grouping all the specimens identified as P. hypochondrialis and specimens assigned P. azurea from Minas Gerais state. This clade is characterized by conserved chromosomal morphology and a common C-banding pattern. Extensive variation in the nucleolar organizing region (NOR) was observed among populations, with four distinct NOR positions being recognized in the karyotypes. Intra-population polymorphism of the additional rDNA clusters observed in specimens from Barreiras, Bahia state, also highlights the marked genomic instability of the rDNA in the genome of this group. Based on the topology obtained in the phylogenetic analyses, the re-evaluation of the taxonomic status of the specimens from the southernmost population known in Brazil is recommended. Conclusions: The results of this study support the need for a thorough revision of the phenotypic features used to discriminate P. azurea and P. hypochondrialis. The phylogenetic data presented here also contribute to an extension of the geographic range of P. hypochondrialis, which is known to occur in the Amazon basin and neighboring areas of the Cerrado savanna, where it may be sympatric with P. azurea, within contact zones. The misidentification of specimens may have led to inconsistencies in the original definition of the geographic range of P. azurea. The variability observed in the NOR of P. hypochondrialis reinforces the conclusion that these sites represent hotspots of rearrangement. Intraspecific variation in the location of these sites is the result of constant rearrangements that are not detected by classical cytogenetic methods or are traits of an ancestral, polymorphic karyotype, which would not be phylogenetically informative for this group. (AU)

Processo FAPESP: 10/11300-7 - Estudos cromossômicos e de sequências de DNA em anuros
Beneficiário:Shirlei Maria Recco-Pimentel
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/17464-1 - Citogenética clássica e molecular e análise do DNA mitocondrial no gênero Phyllomedusa (Anura, Hylidae)
Beneficiário:Daniel Pacheco Bruschi
Linha de fomento: Bolsas no Brasil - Doutorado