The interactions and essential effects of intrinsic insulin-like growth factor-I on Leishmania (Leishmania) major growth within macrophages

Autor(es)

Reis, L. C. ^[1] ; Ramos-Sanchez, E. M. ^[1] ; Goto, H. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, IMTSP, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Med Prevent, Fac Med, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 35, n. 7-8, p. 239-244, JUL 2013.
• Citações Web of Science: 10

Resumo

Previously, we showed in Leishmania infections that extrinsic insulin-like growth factor (IGF)-I favored Leishmania proliferation and leishmaniasis development. In this study, the interaction of intrinsically expressed IGF-I and Leishmania (Leishmania) major in macrophages was addressed, and a key finding was the observation, using confocal microscopy, of the co-localization of IGF-I and parasites within macrophages. Following stimulation with interferon-gamma (IFN-gamma), which is known to inhibit IGF-I production in macrophages, we observed a reduction in the expression of both IGF-I mRNA and protein. This reduced expression was accompanied by a reduction in the cellular parasite load that was completely recovered with the addition of extrinsic IGF-I, which suggests an essential role for IGF-I in Leishmania growth. (AU)

• Processo FAPESP: 08/04106-0 - O papel do fator de crescimento insulina-símile I (IGF-I) na modulação do efeito de citocinas na resistência e suscetibilidade na leishmaniose cutânea murina infectados por Leishmania (L.) major
• Beneficiário: Luiza de Campos Reis
• Modalidade de apoio: Bolsas no Brasil - Doutorado