The eukaryotic translation initiation factor 3 subunit L protein interacts with Flavivirus NS5 and may modulate yellow fever virus replication

Autor(es):Mostrar menos -

Morais, Ana T. S. ^{[1, 2]} ; Terzian, Ana C. B. ^[1] ; Duarte, Danilo V. B. ^[1] ; Bronzoni, Roberta V. M. ^[1] ; Madrid, Maria C. F. S. ^[1] ; Gavioli, Arieli F. ^[1] ; Gil, Laura H. V. G. ^[3] ; Oliveira, Amanda G. ^[3] ; Zanelli, Cleslei F. ^[4] ; Valentini, Sandro R. ^[4] ; Rahal, Paula ^[2] ; Nogueira, Mauricio L. ^{[5, 1, 2]}

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose Rio do Preto FAMERP, Dept Doencas Dermatol Infecciosas & Parasitarias, Lab Pesquisas Virol, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista, Dept Biol, Programa Posgrad Microbiol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Ctr Pesquisas Aggeu Magalhaes CPqAM FIOCRUZ, Dept Virol & Terapia Expt, BR-50670420 Recife, PE - Brazil

^[4] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara - Brazil

^[5] Univ Texas Med Branch, Ctr Trop Dis, Galveston, TX 77555 - USA

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY JOURNAL; v. 10, JUN 22 2013.
• Citações Web of Science: 10

Resumo

Background: Yellow fever virus (YFV) belongs to the Flavivirus genus and causes an important disease. An alarming resurgence of viral circulation and the expansion of YFV-endemic zones have been detected in Africa and South America in recent years. NS5 is a viral protein that contains methyltransferase and RNA-dependent RNA polymerase (RdRp) domains, which are essential for viral replication, and the interactions between NS5 and cellular proteins have been studied to better understand viral replication. The aim of this study was to characterize the interaction of the NS5 protein with eukaryotic translation initiation factor 3 subunit L (eIF3L) and to evaluate the role of eIF3L in yellow fever replication. Methods: To identify interactions of YFV NS5 with cellular proteins, we performed a two-hybrid screen using the YFV NS5 RdRp domain as bait with a human cDNA library, and RNApol deletion mutants were generated and analyzed using the two-hybrid system for mapping the interactions. The RNApol region involved was segmented into three fragments and analyzed using an eIF3L-expressing yeast strain. To map the NS5 residues that are critical for the interactions, we performed site-direct mutagenesis in segment 3 of the interaction domain (ID) and confirmed the interaction using in vitro assays and in vivo coimmunoprecipitation. The significance of eIF3L for YFV replication was investigated using eIF3L overexpression and RNA interference. Results: In this work, we describe and characterize the interaction of NS5 with the translation factor eIF3L. The interaction between NS5 and eIF3L was confirmed using in vitro binding and in vivo coimmunoprecipitation assays. This interaction occurs at a region (the interaction domain of the RNApol domain) that is conserved in several flaviviruses and that is, therefore, likely to be relevant to the genus. eIF3L overexpression and plaque reduction assays showed a slight effect on YFV replication, indicating that the interaction of eIF3L with YFV NS5 may play a role in YFV replication. Conclusions: Although the precise function of eIF3L on interactions with viral proteins is not entirely understood, these results indicate an interaction of eIF3L with YF NS5 and that eIF3L overexpression facilitates translation, which has potential implications for virus replication. (AU)

• Processo FAPESP: 09/01400-7 - Estudo das interações entre a proteína NS4B do vírus da febre amarela e proteínas celulares
• Beneficiário: Maurício Lacerda Nogueira
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05043-1 - Caracterização da interação entre proteínas não estruturais do vírus da Febre Amarela e eIF3L
• Beneficiário: Ana Theresa Silveira de Morais
• Linha de fomento: Bolsas no Brasil - Doutorado Direto