SacRALF1, a peptide signal from the grass sugarcane (Saccharum spp.), is potentially involved in the regulation of tissue expansion

Autor(es)

Mingossi, Fabiana B. ^[1] ; Matos, Juliana L. ^[2] ; Rizzato, Ana Paula ^[1] ; Medeiros, Ane H. ^[1] ; Falco, Maria C. ^[3] ; Silva-Filho, Marcio C. ^[1] ; Moura, Daniel S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, BR-13400970 Piracicaba, SP - Brazil

^[3] CTC, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Molecular Biology; v. 73, n. 3, p. 271-281, June 2010.
• Área do conhecimento: Ciências Biológicas - Bioquímica
• Citações Web of Science: 23
• Assunto(s): Alcalinização Hormônios peptídicos

Resumo

Rapid alkalinization factor (RALF) is part of a growing family of small peptides with hormone characteristics in plants. Initially isolated from leaves of tobacco plants, RALF peptides can be found throughout the plant kingdom and they are expressed ubiquitously in plants. We took advantage of the small gene family size of RALF genes in sugarcane and the ordered cellular growth of the grass sugarcane leaves to gain information about the function of RALF peptides in plants. Here we report the isolation of two RALF peptides from leaves of sugarcane plants using the alkalinization assay. SacRALF1 was the most abundant and, when added to culture media, inhibited growth of microcalli derived from cell suspension cultures at concentrations as low as 0.1 uM. Microcalli exposed to exogenous SacRALF1 for 5 days showed a reduced number of elongated cells. Only four copies of SacRALF genes were found in sugarcane plants. All four SacRALF genes are highly expressed in young and expanding leaves and show a low or undetectable level of expression in expanded leaves. In half-emerged leaf blades, SacRALF transcripts were found at high levels at the basal portion of the leaf and at low levels at the apical portion. Gene expression analyzes localize SacRALF genes in elongation zones of roots and leaves. Mature leaves, which are devoid of expanding cells, do not show considerable expression of SacRALF genes. Our findings are consistent with SacRALF genes playing a role in plant development potentially regulating tissue expansion. (AU)

• Processo FAPESP: 02/08661-1 - Isolamento e caracterização de polipeptpídeos hormonais em cana-de-açúcar
• Beneficiário: Daniel Scherer de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/11109-5 - Identificação e caracterização do receptor do peptídeo hormonal ralf e caracterização da proteína interagente semelhante a calmodulina (cml)
• Beneficiário: Daniel Scherer de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52067-3 - Herbivoria e o transporte intracelular de proteínas
• Beneficiário: Márcio de Castro Silva Filho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático