Investigating the Influence of the Interface in Thiol-Functionalized Silver-Gold Nanoshells over Lipase Activity

Autor(es)

Kisukuri, Camila M. ^[1] ; Macedo, Alexandra ^[2] ; Oliveira, Caio C. S. ^[2] ; Camargo, Pedro H. C. ^[2] ; Andrade, Leandro H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Quim Fina & Biocatalise, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Quim, GrAND Grp Avanco Design Nanomat, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 29, n. 51, p. 15974-15980, DEC 24 2013.
• Citações Web of Science: 5

Resumo

We employed thiol-funcionalized AgAu nanoshells (AgAu NSs) as supports for the covalent attachment of lipases (BCL, Burkholderia cepacia lipase; PPL, pancreatic porcine lipase). Specifically, we were interested in investigating the effect of the nature/size of the spacer in AgAu NSs-functionalized organic thiols over the covalent attachment of lipases. The catalytic performance of AgAu-lipase systems was measured in the kinetic resolution of (R,S)-1-(phenyl)ethanol via a transesterification reaction. In comparison to free BCL, the lipase attached to AgAu NSs using a small spacer such as cysteamine or mercaptoacetic acid, with the largest spacer mercaptoundecanoic acid, had the fastest conversion rate. The recycling potential for BCL was investigated. After three reaction cycles, the enzyme activity was kept at around 90% of the initial value. The results described herein show that the size of the spacer plays an important role in optimizing lipase activities in metallic nanoshells as solid supports. (AU)

• Processo FAPESP: 12/11201-4 - Mudanças na interface de nanopartículas de ouro/prata e lipases: seus efeitos na atividade enzimática
• Beneficiário: Camila de Menezes Kisukuri
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/06847-0 - Nanoestruturas polimetálicas contendo interiores vazios e tamanho, forma e composições definidas
• Beneficiário: Pedro Henrique Cury Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/14196-1 - Reações enzimáticas: explorando o seu potencial como ferramenta sintética
• Beneficiário: Luiz Fernando da Silva Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular