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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Tooth Tissue Engineering: The Influence of Hydrophilic Surface on Nanocrystalline Diamond Films for Human Dental Stem Cells

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Autor(es):
Duailibi, Silvio Eduardo [1, 2, 3] ; Duailibi, Monica Talarico [1, 3] ; Ferreira, Lydia Masako [1] ; Ladislau Carvalho Salmazi, Karina Inacio [4] ; Salvadori, Maria Cecilia [5] ; Teixeira, Fernanda de Sa [5] ; Pasquarelli, Alberto [6] ; Vacanti, Joseph Phillip [7, 8] ; Yelick, Pamela Crotty [9]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, CTCMol, UNIFESP, Dept Med, Div Plast Surg, Ctr Cellular & Mol Therap, BR-04044010 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, Inst Sci & Technol, Sao Jose Dos Campos, SP - Brazil
[3] INCT Biofabris, Nacl Inst Sci & Technol, Biofabricat Inst, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Med, Div Clin Immunol & Allergy, Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil
[6] Univ Ulm, Dept Electron Devices & Circuits, D-89069 Ulm - Germany
[7] Massachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 - USA
[8] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 - USA
[9] Tufts Univ, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 - USA
Número total de Afiliações: 9
Tipo de documento: Artigo Científico
Fonte: TISSUE ENGINEERING PART A; v. 19, n. 23-24, p. 2537-2543, DEC 1 2013.
Citações Web of Science: 7
Resumo

New techniques for tissue engineering (TE) are rapidly emerging. The basic concept of autologous TE is to isolate cells from small biopsy specimens, and to expand these cells in culture for subsequent seeding onto biodegradable scaffolds. Nanocrystalline diamond films have attracted the attention of researchers from a variety of different areas in recent years, due to their unique and exceptional properties. In this approach, human dental stem cells (hDSCs) were characterized by flow cytometry and grown on diamond films with hydrogen (H)-terminated and oxygen (O)-terminated surfaces for 28 days, and then removed by lysis and washing with distilled water. Energy dispersive spectroscopy analysis was performed, showing that the regions with O-terminated surfaces contained much higher levels of deposited calcium, oxygen, and phosphorus. These results suggest that the extracellular matrix was considerably more developed in the O-terminated regions, as compared with the H-terminated regions. In addition, optical microscopy of hDSCs cultured on the diamond substrate with H- and O-terminated surfaces, before washing with distilled water, showed preferential directions of the cells arrangement, where orthogonal lines suggest that the cells appeared to be following the O-terminated regions or hydrophilic surface. These findings suggest that O-terminated diamond surfaces prepared on biodegradable scaffolds can be useful for mineralized dental tissue formation. (AU)

Processo FAPESP: 08/57860-3 - BIOFABRIS - Instituto de Biofabricação
Beneficiário:Rubens Maciel Filho
Linha de fomento: Auxílio à Pesquisa - Temático