Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Unravelling the Neospora caninum secretome through the secreted fraction (ESA) and quantification of the discharged tachyzoite using high-resolution mass spectrometry-based proteomics

Texto completo
Autor(es):
Pollo-Oliveira, Leticia [1, 2] ; Post, Harm [3, 4, 5] ; Acencio, Marcio Luis [6] ; Lemke, Ney [6] ; van den Toorn, Henk [3, 4, 5] ; Tragante, Vinicius [7, 8] ; Heck, Albert J. R. [3, 4, 5] ; Altelaar, A. F. Maarten [3, 4, 5] ; Yatsuda, Ana Patricia [1, 2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Nucleo Apoio Pesquisa Prod Nat Sintet NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3884 CH Utrecht - Netherlands
[4] Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3884 CH Utrecht - Netherlands
[5] Netherlands Prote Ctr, NL-3884 CH Utrecht - Netherlands
[6] Univ Estadual Paulista, UNESP, Botucatu Inst Biosci, BR-18918970 Botucatu, SP - Brazil
[7] Univ Med Ctr Utrecht, Dept Cardiol, Div Heart & Lungs, Utrecht - Netherlands
[8] Univ Med Ctr Utrecht, Dept Med Genet, Div Biomed Genet, Utrecht - Netherlands
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: PARASITES & VECTORS; v. 6, NOV 23 2013.
Citações Web of Science: 9
Resumo

Background: The apicomplexan parasite Neospora caninum causes neosporosis, a disease that leads to abortion or stillbirth in cattle, generating an economic impact on the dairy and beef cattle trade. As an obligatory intracellular parasite, N. caninum needs to invade the host cell in an active manner to survive. The increase in parasite cytosolic Ca2+ upon contact with the host cell mediates critical events, including the exocytosis of phylum-specific secretory organelles and the activation of the parasite invasion motor. Because invasion is considered a requirement for pathogen survival and replication within the host, the identification of secreted proteins (secretome) involved in invasion may be useful to reveal interesting targets for therapeutic intervention. Methods: To chart the currently missing N. caninum secretome, we employed mass spectrometry-based proteomics to identify proteins present in the N. caninum tachyzoite using two different approaches. The first approach was identifying the proteins present in the tachyzoite-secreted fraction (ESA). The second approach was determining the relative quantification through peptide stable isotope labelling of the tachyzoites submitted to an ethanol secretion stimulus (discharged tachyzoite), expecting to identify the secreted proteins among the down-regulated group. Results: As a result, 615 proteins were identified at ESA and 2,011 proteins quantified at the discharged tachyzoite. We have analysed the connection between the secreted and the down-regulated proteins and searched for putative regulators of the secretion process among the up-regulated proteins. An interaction network was built by computational prediction involving the up-and down-regulated proteins. The mass spectrometry proteomics data have been deposited to the ProteomeXchange with identifier PXD000424. Conclusions: The comparison between the protein abundances in ESA and their measure in the discharged tachyzoite allowed for a more precise identification of the most likely secreted proteins. Information from the network interaction and up-regulated proteins was important to recognise key proteins potentially involved in the metabolic regulation of secretion. Our results may be helpful to guide the selection of targets to be investigated against Neospora caninum and other Apicomplexan organisms. (AU)

Processo FAPESP: 10/20684-3 - Utilização de aprendizado de máquina em redes biológicas para previsão e determinação de regras para emergência de fenótipos de interesse
Beneficiário:Marcio Luis Acencio
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/02018-4 - Aprendizado de máquina em biologia molecular de sistemas (AMBiS) aplicação em letalidade sintética, genes condicionalmente essenciais e transcrição gênica cooperativa
Beneficiário:Ney Lemke
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 05/53785-9 - Proteínas envolvidas na invasão de células hospedeiras por protozoários apicomplexas como potenciais componentes para métodos profiláticos
Beneficiário:Ana Patricia Yatsuda Natsui
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores