Use of Adipose Tissue-Derived Mesenchymal Stem Cells for Experimental Tendinitis Therapy in Equines

Autor(es)

Carvalho, Armando de Mattos ^[1] ; Garcia Alves, Ana Liz ; Gomes de Oliveira, Patricia Galvao ; Cisneros Alvarez, Luis Emiliano ^[2] ; Amorim, Renee Laufer ^[3] ; Hussni, Carlos Alberto ; Deffune, Elenice ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Vet Surg & Anesthesiol, Sch Vet Med & Anim Sci, UNESP, BR-560 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci, BR-560 Botucatu, SP - Brazil

^[3] Sao Paulo State Univ, Dept Clin Vet, Sch Vet Med & Anim Sci, BR-560 Botucatu, SP - Brazil

^[4] Sao Paulo State Univ, Ctr Blood, Botucatu Med Sch, BR-560 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Equine Veterinary Science; v. 31, n. 1, p. 26-34, Jan. 2011.
• Área do conhecimento: Ciências Agrárias - Medicina Veterinária
• Citações Web of Science: 52
• Assunto(s): Equinos Tendinite animal Células-tronco mesenquimais Terapia baseada em transplante de células e tecidos Enzimas Colagenases

Resumo

Superficial digital flexor tendon lesion is an important cause of lameness in equine athletes. Although numerous treatments have been described, few are effective at promoting significant improvement in the quality of the extracellular matrix. Therefore, great potential remains for recurrence and in certain cases, an abrupt end to the horse’s athletic career. Recently, several experiments have focused on the therapeutic potential of mesenchymal stem cells (MSCs) in cases of tendon lesions. This study aimed to evaluate the effect of adipose tissue-derived MSCs in the treatment of induced tendinitis of the superficial digital flexor tendon in horses by clinical, ultrasonographic, histopathological, and immunochemical analyses. Tendinitis was induced in both thoracic limbs of eight mares by administration of collagenase solution and adipose tissue was collected from the tail base for MSCs isolation and expansion, which were used during cellular therapy on only one limb 30 days after lesion induction. No differences occurred between the groups regarding the clinical and ultrasonographic analyses; however, histopathological evaluation revealed a significant improvement in tendon fiber organization and diminished inflammatory infiltrate, whereas immunohistochemical analysis showed increased expression of type I collagen in the treated group as compared with controls. The cellular therapy model implanted in this experiment promoted increased perivascular inflammatory infiltrate, fibroblastic density, neovascularization, and qualitative healing improvement of tendon extracellular matrix, in terms of fiber orientation and type I/III collagen ratio; moreover, it was considered to be a safe and viable process. (AU)

• Processo FAPESP: 07/53496-2 - Implante autólogo de células estromais do tecido adiposo no tratamento de tendinites experimentais em equinos: avaliação clínica, ultrassonográfica, histopatológica e imunoistoquímica
• Beneficiário: Armando de Mattos Carvalho
• Modalidade de apoio: Bolsas no Brasil - Mestrado