| Texto completo | |
| Autor(es): |
Fontana, Karina
[1, 2]
;
Campos, Gerson E. R.
[3]
;
Staron, Robert S.
[4]
;
da Cruz-Hoefling, Maria Alice
[2]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, Campinas - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Histol & Embryol, Campinas - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Estrutural & Func, Campinas - Brazil
[4] Ohio Univ, Dept Biomed Sci, Heritage Coll Osteopath Med, Athens, OH 45701 - USA
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 8, n. 11 NOV 15 2013. |
| Citações Web of Science: | 6 |
| Resumo | |
In an attempt to shorten recovery time and improve performance, strength and endurance athletes occasionally turn to the illicit use of anabolic-androgenic steroids (AAS). This study evaluated the effects of AAS treatment on the muscle mass and phenotypic characteristics of transgenic mice subjected to a high-intensity, aerobic training program (5d/wk for 6 weeks). The transgenic mice (CETP+/-LDLr-/+) were engineered to exhibit a lipid profile closer to humans. Animals were divided into groups of sedentary (Sed) and/or training (Ex) mice (each treated orally with AAS or gum arabic/vehicle: Sed-C, Sed-M, ex-C, ex-M). The effects of AAS (mesterolone: M) on specific phenotypic adaptations (muscle wet weight, cross-sectional area, and fiber type composition) in three hindlimb muscles (soleus: SOL, tibialis anterior: TA and gastrocnemius: GAS) were assessed. In order to detect subtle changes in fiber type profile, the entire range of fiber types (I, IC, IIAC, IIA, IIAD, IID, IIDB, IIB) was delineated using mATPase histochemistry. Body weight gain occurred throughout the study for all groups. However, the body weight gain was significantly minimized with exercise. This effect was blunted with mesterolone treatment. Both AAS treatment (Sed-M) and high-intensity, aerobic training (ex-C) increased the wet weights of all three muscles and induced differential hypertrophy of pure and hybrid fibers. Combination of AAS and training (ex-M) resulted in enhanced hypertrophy. In the SOL, mesterolone treatment (Sed-M and ex-M) caused dramatic increases in the percentages of fiber types IC, IIAC, IIAD, IID, with concomitant decrease in IIA, but had minimal impact on fiber type percentages in the predominantly fast muscles. Overall, the AAS-induced differential adaptive changes amounted to significant fiber type transformations in the fast-to-slow direction in SOL. AAS treatment had a significant effect on muscle weights and fiber type composition in SOL, TA and GAS which was even maximized in animals subjected to metabolically high-intensity aerobic exercise. (AU) | |
| Processo FAPESP: | 04/13767-9 - Influência dos esteroides anabólico-androgênicos (EAA) associados ao exercício físico sobre os músculos de camundongos que expressam proteína de transferência de éster de colesteril (CETP) com deficiência parcial do receptor de LDL-colesterol |
| Beneficiário: | Maria Alice da Cruz Hofling |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/13768-5 - Influência dos esteróides anabólico-androgênicos (EAA) associados ao exercício físico sobre os músculos de camundongos que expressam proteína de transferência de éster de colesteril (CETP) com deficiência parcial do receptor de LDL-colesterol |
| Beneficiário: | Karina Fontana |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |