Involvement of microRNA-related regulatory pathways in the glucose-mediated control of Arabidopsis early seedling development

Autor(es)

Duarte, Gustavo Turqueto ^[1] ; Matiolli, Cleverson Carlos ^[1] ; Pant, Bikram Datt ^{[2, 3]} ; Schlereth, Armin ^[2] ; Scheible, Wolf-Ruediger ^{[2, 3]} ; Stitt, Mark ^[2] ; Vicentini, Renato ^[4] ; Vincentz, Michel ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Lab Genet Plantas, BR-13083875 Campinas, SP - Brazil

^[2] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam - Germany

^[3] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 - USA

^[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Lab Bioinformat & Biol Sistemas, BR-13083875 Campinas, SP - Brazil

^[5] Inst Biol, Dept Biol Vegetal, BR-13083875 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Experimental Botany; v. 64, n. 14, p. 4301-4312, NOV 2013.
• Citações Web of Science: 9

Resumo

In plants, sugars such as glucose act as signalling molecules that promote changes in gene expression programmes that impact on growth and development. Recent evidence has revealed the potential importance of controlling mRNA decay in some aspects of glucose-mediated regulatory responses suggesting a role of microRNAs (miRNAs) in these responses. In order to get a better understanding of glucose-mediated development modulation involving miRNA-related regulatory pathways, early seedling development of mutants impaired in miRNA biogenesis (hyl1-2 and dcl1-11) and miRNA activity (ago1-25) was evaluated. All mutants exhibited a glucose hyposensitive phenotype from germination up to seedling establishment, indicating that miRNA regulatory pathways are involved in the glucose-mediated delay of early seedling development. The expression profile of 200 miRNA primary transcripts (pri-miRs) was evaluated by large-scale quantitative real-time PCR profiling, which revealed that 38 pri-miRs were regulated by glucose. For several of them, the corresponding mature miRNAs are known to participate directly or indirectly in plant development, and their accumulation was shown to be co-regulated with the pri-miR by glucose. Furthermore, the expression of several miRNA target genes was found to be deregulated in response to glucose in the miRNA machinery mutants ago1-25, dcl1-11, and hyl1-2. Also, in these mutants, glucose promoted misexpression of genes for the three abscisic acid signalling elements ABI3, ABI4, and ABI5. Thus, miRNA regulatory pathways play a role in the adjustments of growth and development triggered by glucose signalling. (AU)

• Processo FAPESP: 08/54529-4 - Avaliação da importância do controle da estabilidade de RNAm na sinalização por glicose e na interação dos sinais glicose e ABA em Arabidopsis thaliana
• Beneficiário: Gustavo Turqueto Duarte
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/52071-0 - Homeostase energética e sinalização por açúcar: diversificação dos mecanismos moleculares envolvidos no controle do balanço energético em angioespermas
• Beneficiário: Michel Georges Albert Vincentz
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático