Molecular characterization, serotyping, and antibiotic susceptibility profile of Leptospira interrogans serovar Copenhageni isolates from Brazil

Autor(es):Mostrar menos -

Miraglia, Fabiana ^[1] ; Matsuo, Minekazo ^[2] ; Morais, Zenaide Maria ^[1] ; Dellagostin, Odir Antonio ^[3] ; Seixas, Fabiana Koemmling ^[3] ; Freitas, Julio Cesar ^[4] ; Hartskeerl, Rudy ^{[5, 6]} ; Moreno, Luisa Zanolli ^[1] ; Costa, Barbara Leticia ^[1] ; Souza, Gisele Oliveira ^[1] ; Vasconcellos, Silvio Arruda ^[1] ; Moreno, Andrea Micke ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil

^[2] Prefeitura Municipio Sao Paulo, Unidade Vigilancia Saude, Sao Paulo, SP - Brazil

^[3] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol Biotecnol, Pelotas, RS - Brazil

^[4] Univ Estadual Londrina, Lab Leptospirose Anim, Londrina, PR - Brazil

^[5] Royal Trop Inst, KIT Biomed Res, WHO, FAO, OIE, NL-1105 AZ Amsterdam - Netherlands

^[6] Royal Trop Inst, KIT Biomed Res, Natl Leptospirosis Reference Ctr, NL-1105 AZ Amsterdam - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 77, n. 3, p. 195-199, NOV 2013.
• Citações Web of Science: 20

Resumo

Leptospira interrogans serogroup Icterohaemorrhagiae is the major serogroup infecting humans worldwide, and rodents and dogs are the most significant transmission sources in urban environments. Knowledge of the prevalent serovars and their maintenance hosts is essential to understand the epidemiology of leptospirosis. In this study, 20 Leptospira isolates were evaluated by pulsed-field gel electrophoresis (PFGE), variable number tandem-repeat analysis (VNTR), serotyping, and determination of antimicrobial resistance profile. Isolates, originated from bovine, canine, human, and rodent sources, were characterized by microscopic agglutination test with polyclonal and monoclonal antibodies and were identified as L. interrogans serogroup Icterohaemorrhagiae serovar Copenhageni. MICs of antimicrobials often used in veterinary medicine were determined by broth microdilution test. Most of tested antibiotics were effective against isolates, including penicillin, ampicillin, and ceftiofur. Higher MIC variability was observed for fluoroquinolones and neomycin; all isolates were resistant to trimethoprim/sulfamethoxazole and sulphadimethoxine. Isolates were genotyped by PFGE and VNTR; both techniques were unable to discriminate between serovars Copenhageni and Icterohaemorrhagiae, as expected. PFGE clustered all isolates in 1 pulsotype, indicating that these serovars can be transmitted between species and that bovine, rodent, and dogs can maintain them in the environment endangering the human population. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/18290-0 - Caracterização de estirpes de Leptospira isoladas no Brasil através da tipagem por sequenciamento de multilocus (MLST)
• Beneficiário: Andrea Micke Moreno
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/52925-2 - Estudo comparativo de procedimentos destinados ao controle da potencia de bacterinas anti-leptospirose suina.
• Beneficiário: Silvio Arruda Vasconcellos
• Modalidade de apoio: Auxílio à Pesquisa - Regular