Antifungal activity of silver nanoparticles in combination with nystatin and chlorhexidine digluconate against Candida albicans and Candida glabrata biofilms

Autor(es)

Monteiro, Douglas R. ^{[1, 2]} ; Silva, Sonia ^[2] ; Negri, Melyssa ^{[3, 2]} ; Gorup, Luiz F. ^[4] ; de Camargo, Emerson R. ^[4] ; Oliveira, Rosario ^[2] ; Barbosa, Debora B. ^[1] ; Henriques, Mariana ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Dent Mat & Prosthodont, Aracatuba Dent Sch, Sao Paulo - Brazil

^[2] Univ Minho, Dept Biol Engn, Inst Biotechnol & Bioengn, P-4710057 Braga - Portugal

^[3] Fac Inga, Maringa, Parana - Brazil

^[4] Fed Univ Sao Carlos UFSCar, LIEC Dept Chem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MYCOSES; v. 56, n. 6, p. 672-680, NOV 2013.
• Citações Web of Science: 33

Resumo

Although silver nanoparticles (SN) have been investigated as an alternative to conventional antifungal drugs in the control of Candida-associated denture stomatitis, the antifungal activity of SN in combination with antifungal drugs against Candida biofilms remains unknown. Therefore, the aim of this study was to evaluate the antifungal efficacy of SN in combination with nystatin (NYT) or chlorhexidine digluconate (CHG) against Candida albicans and Candida glabrata biofilms. The drugs alone or combined with SN were applied on mature Candida biofilms (48h), and after 24h of treatment their antibiofilm activities were assessed by total biomass quantification (by crystal violet staining) and colony forming units enumeration. The structure of Candida biofilms was analysed by scanning electron microscopy (SEM) images. The data indicated that SN combined with either NYT or CHG demonstrated synergistic antibiofilm activity, and this activity was dependent on the species and on the drug concentrations used. SEM images showed that some drug combinations were able to disrupt Candida biofilms. The results of this study suggest that the combination of SN with NYT or CHG may have clinical implications in the treatment of denture stomatitis. However, further studies are needed before recommending the use of these drugs safely in clinical situations. (AU)

• Processo FAPESP: 09/15146-5 - Análise da ação de nanopartículas de prata sobre o biofilme de espécies de Candida
• Beneficiário: Douglas Roberto Monteiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado