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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

NEUROPROTECTIVE EFFECTS OF MESENCHYMAL STEM CELLS ON SPINAL MOTONEURONS FOLLOWING VENTRAL ROOT AXOTOMY: SYNAPSE STABILITY AND AXONAL REGENERATION

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Autor(es):
Spejo, A. B. [1] ; Carvalho, J. L. [2] ; Goes, A. M. [2] ; Oliveira, A. L. R. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP - Brazil
[2] ICB UFMG, Dept Biochem & Immunol, Belo Horizonte, MG - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Neuroscience; v. 250, p. 715-732, OCT 10 2013.
Citações Web of Science: 29
Resumo

Compression of spinal roots is an important medical problem, which may arise from intervertebral disc herniation, tumor growth or as a result of high energy accidents. Differently from avulsion, root crushing maintains the central/peripheral nervous system (CNS/PNS) connection, although the axons are axotomized and motoneurons degenerate. Such neuronal death may decrease and delay motor function recovery. In the present study we have investigated the neuroprotective effects of mesenchymal stem cell (MSC) therapy following such proximal lesions. Motor recovery and synaptic stabilization were analyzed by the use of morphological and functional approaches. For that, crushing the ventral roots at L4, L5 and L6 was unilaterally performed in Lewis rats. Four weeks after injury, an increased motoneuron survival was observed in the MSCtreated group, coupled with a smaller decrease of inputs at the motoneuron surface and nearby neuropil, seen by synaptophysin and synapsin immunolabeling and decreased astrogliosis, seen by GFAP immunolabeling. In this sense, MSC-treated group displayed a significant preservation of GABAergic terminals, indicating a possible neuroprotection to glutamate excitotoxicity. Motor function recovery was acutely improved in MSC-treated group as compared to Dulbeco's modified eagle medium (DMEM)-treated. Overall, we provide evidence that ventral root crushing (VRC), although milder than avulsion, results in significant loss of motoneurons (similar to 51%) that can be reduced by MSC administration within the spinal cord. Such treatment also improves the number of synapses immunoreactive against molecules present in inhibitory inputs. Also, an increased number of regenerated axons was obtained in the MSC-treated group, in comparison to the DMEM-treated control. Overall, MSC therapy acutely improved limb strength and gait coordination, indicating a possible clinical application of such treatment following proximal lesions at the CNS/PNS interface. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/14210-9 - Regeneração nervosa após esmagamento de raízes motoras na interface do SNC e SNP e tratamento com células tronco mesenquimais
Beneficiário:Aline Barroso Spejo
Linha de fomento: Bolsas no Brasil - Mestrado