Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates

Autor(es)

Correa Fehlberg, Lorena Cristina ^[1] ; Sales Andrade, Lucas Henrique ^[1] ; Assis, Diego Magno ^[2] ; Vicente Pereira, Rosana Helena ^[3] ; Gales, Ana Cristina ^[1] ; Marques, Elizabeth Andrade ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Lab Alerta, Sao Paulo - Brazil

^[2] Bruker Brasil, Sao Paulo - Brazil

^[3] Univ Estado Rio de Janeiro, Fac Ciencias Med, Dept Microbiol Imunol & Parasitol, BR-20550011 Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 77, n. 2, p. 126-128, OCT 2013.
• Citações Web of Science: 26

Resumo

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) for identification of Bcc species compared with that of recA sequencing. MALDI-ToF was able of identifying 100% of Bcc isolates at the genus level, but 23.1% of Bcc isolates tested were not correctly identified at the species level. The misidentification occurred most frequently with Burkholderia contaminans (100%) and B. cepacia (33.3%). (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/04910-9 - Complexo Burkholderia cepacia: caracterização do perfil de sensibilidade a antimicrobianos e avaliação dos mecanismos de resistência a sulfametoxazol/trimetoprim
• Beneficiário: Ana Cristina Gales
• Modalidade de apoio: Auxílio à Pesquisa - Regular