Differential arthritogenicity of Staphylococcus aureus strains isolated from biological samples

Autor(es)

Colavite-Machado, Priscila Maria ^[1] ; Watanabe Ishikawa, Larissa Lumi ^[1] ; Donega Franca, Thais Graziela ^[1] ; Goncalves Zorzella-Pezavento, Sofia Fernanda ^[1] ; da Rosa, Larissa Camargo ^[1] ; Chiuso-Minicucci, Fernanda ^[1] ; Ribeiro de Souza da Cunha, Maria de Lourdes ^[1] ; Garlet, Gustavo Pompermaier ^[2] ; Sartori, Alexandrina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618070 Sao Paulo - Brazil

^[2] Sao Paulo Univ FOB USP, Dept Biol Sci, Sch Dent Bauru, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC INFECTIOUS DISEASES; v. 13, AUG 30 2013.
• Citações Web of Science: 5

Resumo

Background: Staphylococcus aureus is the most common agent of septic arthritis that is a severe, rapidly progressive and destructive joint disease. Superantigens produced by S. aureus are considered the major arthritogenic factors. In this study, we compared the arthritogenic potential of five superantigen-producing staphylococcal strains. Methods: Male C57BL/6 mice were intravenously infected with ATCC 19095 SEC+, N315 ST5 TSST-1(+), S-70 TSST-1(+), ATCC 51650 TSST-1(+) and ATCC 13565 SEA(+) strains. Clinical parameters as body weight, arthritis incidence and clinical score were daily evaluated. Joint histopathological analysis and spleen cytokine production were evaluated at the 14th day after infection. Results: Weight loss was observed in all infected mice. ATCC 19095 SEC+, N315 ST5 TSST-1(+) and S-70 TSST-1(+) were arthritogenic, being the highest scores observed in ATCC 19095 SEC+ infected mice. Intermediate and lower clinical scores were observed in N315 ST5 TSST-1(+) and S-70 TSST-1(+) infected mice, respectively. The ATCC 13565 SEA(+) strain caused death of 85% of the animals after 48 h. Arthritis triggered by the ATCC 19095 SEC+ strain was characterized by accentuated synovial hyperplasia, inflammation, pannus formation, cartilage destruction and bone erosion. Similar joint alterations were found in N315 ST5 TSST-1(+) infected mice, however they were strikingly more discrete. Only minor synovial proliferation and inflammation were triggered by the S-70 TSST-1(+) strain. The lowest levels of TNF-alpha, IL-6 and IL-17 production in response to S. aureus stimulation were found in cultures from mice infected with the less arthritogenic strains (S-70 TSST-1(+) and ATCC 51650 TSST-1(+)). The highest production of IL-17 was detected in mice infected with the most arthritogenic strains (ATCC 19095 SEC+ and N315 ST5 TSST-1(+)). Conclusions: Together these results demonstrated that S. aureus strains, isolated from biological samples, were able to induce a typical septic arthritis in mice. These results also suggest that the variable arthritogenicity of these strains was, at least in part, related to their differential ability to induce IL-17 production. (AU)

• Processo FAPESP: 11/04323-3 - Impacto do uso de droga imunomoduladora no tratamento da artrite séptica por Staphylococcusa aureus
• Beneficiário: Priscila Maria Colavite Machado
• Modalidade de apoio: Bolsas no Brasil - Mestrado