A low-cost automated flow analyzer based on low temperature co-fired ceramic and LED photometer for ascorbic acid determination

Autor(es)

Pessoa-Neto, Osmundo D. ^[1] ; dos Santos, Vagner B. ^[1] ; Vicentini, Fernando C. ^[1] ; Suarez, Willian T. ^[2] ; Alonso-Chamarro, Julian ^[3] ; Fatibello-Filho, Orlando ^[1] ; Faria, Ronaldo C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Vicosa, Exact & Technol Sci Ctr, Dept Chem, BR-36570000 Vicosa, MG - Brazil

^[3] Autonomous Univ Barcelona, Dept Chem, Grp Sensors & Biosensors, Bellaterra 08193 - Spain

• Tipo de documento: Artigo Científico
• Fonte: CENTRAL EUROPEAN JOURNAL OF CHEMISTRY; v. 12, n. 3, p. 341-347, MAR 2013.
• Citações Web of Science: 5

Resumo

An automated flow analyzer based on low temperature co-fired ceramic (LTCC), a solid-phase reactor (SPR) and a low-cost photometer was designed for ascorbic acid (AA) determination in pharmaceutical formulations. It consists of a peristaltic pump, three-way solenoid valves, SPR to chemically convert Cu(II) into Cu(I), and a LTCC device for mixing the liberated copper with bathocuproine and detection. The flow cell in the LTCC employed an ultrabright LED - photodiode photometer. The analyzer successfully determined AA in pharmaceutical formulations. The analytical curve from 8.5x10(-6) to 7.0x10(-4) M gave a detection limit of 7.0x10(-7) M and a RSD of 2.1% for a 2.0x10(-4) M AA solution (n = 10). A high sampling frequency of 102 h(-1) and low reagent and sample consumption (150 A mu L) resulted. (AU)

• Processo FAPESP: 11/02259-6 - Desenvolvimento de biossensores para determinação de biomarcadores proteicos visando à aplicação no diagnóstico precoce e monitoramento de câncer
• Beneficiário: Ronaldo Censi Faria
• Linha de fomento: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 08/06867-8 - Desenvolvimento, caracterização e aplicação analítica de eletrodos nanocompósitos na determinação de catecolaminas e flavonóides
• Beneficiário: Ronaldo Censi Faria
• Linha de fomento: Auxílio à Pesquisa - Regular