Cytotoxic Effects of Zoledronic Acid on Human Epithelial Cells and Gingival Fibroblasts

Autor(es)

Fernanda Goncalves Basso ^[1] ; Taisa Nogueira Pansani ^[2] ; Camila Favero de Oliveira ^[3] ; Ana Paula Silveira Turrioni ^[4] ; Diana Gabriela Soares ^[5] ; Josimeri Hebling ^[6] ; Carlos Alberto de Souza Costa ^[7]

Afiliação do(s) autor(es)

^[1] Piracicaba School of Dentistry. UNICAMP University of Campinas - Brasil

^[2] Araraquara School of Dentistry. UNESP - Univ Estadual Paulista - Brasil

^[3] Butantan Institute/Departament of Morphology of UNIFESP - Federal University of Sao Paulo. Laboratory of Genetics - Brasil

^[4] Araraquara School of Dentistry. UNESP - Univ Estadual Paulista - Brasil

^[5] Araraquara School of Dentistry. UNESP - Univ Estadual Paulista - Brasil

^[6] Araraquara School of Dentistry. UNESP - Univ Estadual Paulista - Brasil

^[7] Araraquara School of Dentistry. UNESP - Univ Estadual Paulista - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 24, n. 6, p. 551-558, 2013-12-00.

Resumo

Bisphosphonate-induced osteonecrosis has been related to the cytotoxicity of these drugs on oral mucosa cells. A previous study showed that 5 µM of zoledronic acid (ZA), a nitrogen-containing bisphosphonate, is the highest concentration of this drug found in the oral cavity of patients under treatment. Therefore, in order to simulate an osteonecrosis clinical condition, the aim of this study was to evaluate the highest concentration of ZA applied on human epithelial cells (HaCaT) and gingival fibroblasts. For this purpose, cells (3×104 cells/cm2) were seeded in wells for 48 h using complete culture medium (cDMEM). After 48 h incubation, the cDMEM was replaced by fresh serum-free culture medium (DMEM-FBS) in which the cells were maintained for additional 24 h. Then, 5 µM ZA were added to the DMEM–FBS and the cells incubated in contact with the drug for 48 h. After this period, the number of viable cells (trypan blue), cell viability (MTT assay), total protein (TP) production and cell morphology (SEM analysis) were assessed. Data were analyzed statistically by Mann-Whitney, ANOVA and Tukey's test (α=0.05). ZA caused a significant reduction in the number of viable cells and decreased the metabolic activity of both cell lines. However, decrease of TP production occurred only in the epithelial cell cultures. Morphological alterations were observed in both cell types treated with ZA. In conclusion, ZA (5 µM) was cytotoxic to human epithelial cells and gingival fibroblast cultures, which could be associated, clinically, with the development of bisphosphonate-induced osteonecrosis. (AU)

• Processo FAPESP: 09/52326-1 - Avaliação in vitro dos efeitos da laserterapia de baixa intensidade sobre cultura de osteoblastos, fibroblastos e queratinócitos submetidos ao tratamento com ácido zoledrônico
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/54722-1 - Avaliação in vitro dos efeitos da laserterapia de baixa intensidade sobre cultura de osteoblastos, fibroblastos e queratinócitos submetidos ao tratamento com ácido zoledrônico
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular