Clavulanic acid production by the MMS 150 mutant obtained from wild type Streptomyces clavuligerus ATCC 27064

Autor(es)

Eliton da Silva Vasconcelos ^[1] ; Vanderlei Aparecido de Lima ^[1] ; Leandro Seiji Goto ^{[1, 2]} ; Isara Lourdes Cruz-Hernández ^[4] ; Carlos Osamu Hokka ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Engn Quim, BR-13560 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis Sao Carlos, Grp Biofis Mol Sergio Mascarenhas, Sao Carlos, SP - Brazil

^[3] Universidade Federal de São Carlos. Departamento de Engenharia Química - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 44, n. 4, p. 1049-1057, 2014-01-24.

Resumo

Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive metabolites. However, parental strains are susceptible to loss of their original productivity due genetic instability phenomenona. In this work, some S. clavuligerus mutant strains obtained by treatment with UV light and with MMS are compared with the wild type (Streptomyces clavuligerus ATCC 27064). The results indicated that the random mutations originated some strains with different phenotypes, most divergent demonstrated by the mutants strains named AC116, MMS 150 and MMS 54, that exhibited lack of pigmentation in their mature spores. Also, the strain MMS 150 presented a larger production of CA when cultivated in semi-synthetics media. Using other media, the wild type strain obtained a larger CA production. Besides, using the modifed complex media the MMS 150 strain showed changes in its lipolitic activity and a larger production of CA. The studies also allowed finding the best conditions for a lipase activity exhibited by wild type S. clavuligerus and the MMS150 mutant. (AU)

• Processo FAPESP: 06/59693-1 - Caracterização de produtos gênicos pertinentes à via de síntese do ácido clavulânico de Streptomyces clavuligerus ATCC 27064
• Beneficiário: Leandro Seiji Goto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado