Titanium and zirconia particle-induced pro-inflammatory gene expression in cultured macrophages and osteolysis, inflammatory hyperalgesia and edema in vivo

Autor(es)

Obando-Pereda, G. A. ^[1] ; Fischer, L. ^[2] ; Stach-Machado, D. R. ^[1]

Afiliação do(s) autor(es)

^[1] IB UNICAMP, Inst Biol, Dept Anat Cell Biol Physiol & Biophys, Lab Appl Immunol, Campinas, SP - Brazil

^[2] Univ Fed Parana, Dept Physiol, Lab Pain Physiol, BR-80060000 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 97, n. 2, p. 96-106, MAR 3 2014.
• Citações Web of Science: 27

Resumo

Aims: The biological reaction to wear debris is critical to the osteolysis underlying aseptic loosening of joint prosthetic implants. In an attempt to reduce aseptic loosening, ceramics have been introduced. This study was designed to evaluate, compare and correlate the expression of Toll-like receptors (TLRs), their intracellular adaptors and proinflammatory cytokines in cultured macrophages challenged with titanium or zirconia particles, as well as particle-induced osteolysis in calvaria and hyperalgesia and edema in hind paw. Main methods: TLRs and their adaptors were evaluated at the mRNA level by RT-PCR, and cytokine expression was evaluated at the mRNA and protein levels. Osteolysis and hyperalgesia and edema were evaluated in vivo, in calvaria and hind paw, respectively. Key findings: Cultured macrophages challenged with zirconia or titanium particles expressed increased mRNA for TLRs 2, 3, 4 and 9, and their adaptors MyD88, TR1F and NF-kappa B and cytokines TNF-alpha, IL-1 beta and 1L-6, which were also increased at protein level. Quantitative differences are evident and, in general, zirconia particle-induced pro-inflammatory gene expression was lower than that induced by titanium particles. In in vivo experiments, exposition to titanium or zirconia particles induced osteolysis in calvaria and hyperalgesia and edema in hind paw; however those induced by zirconia particles were significantly lower. There is a strong and positive correlation between the expressions of mRNA for TLR4, NF-kappa B, TNF-alpha, IL-1 beta and IL-6. Significance: Collectively, our data suggest that zirconia ceramic particles are less bioactive than titanium particles. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/58796-4 - Importancia e funcao dos receptores tlr-2 e r no reconhecimento da zirconia e titanio.
• Beneficiário: Gustavo Alberto Obando Pereda
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/56731-2 - Função e envolvimento dos receptores TLR2 e TLR4 no reconhecimento das partículas de titânio e zircônia
• Beneficiário: Dagmar Ruth Stach - Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular