Phylogeny of culturable cyanobacteria from Brazilian mangroves

Autor(es)

Pamplona Silva, Caroline Souza ^[1] ; Genuario, Diego Bonaldo ^[1] ; Marcal Vieira Vaz, Marcelo Gomes ^[1] ; Fiore, Marli Fatima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Lab Mol Ecol Cyanobacteria, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Systematic and Applied Microbiology; v. 37, n. 2, p. 100-112, MAR 2014.
• Citações Web of Science: 29

Resumo

The cyanobacterial community from Brazilian mangrove ecosystems was examined using a culture-dependent method. Fifty cyanobacterial strains were isolated from soil, water and periphytic samples collected from Cardoso Island and Bertioga mangroves using specific cyanobacterial culture media. Unicellular, homocytous and heterocytous morphotypes were recovered, representing five orders, seven families and eight genera (Synechococcus, Cyanobium, Cyanobacterium, Chlorogloea, Leptolyngbya, Phormidium, Nostoc and Microchaete). All of these novel mangrove strains had their 16S rRNA gene sequenced and BLAST analysis revealed sequence identities ranging from 92.5 to 99.7% when they were compared with other strains available in GenBank. The results showed a high variability of the 16S rRNA gene sequences among the genotypes that was not associated with the morphologies observed. Phylogenetic analyses showed several branches formed exclusively by some of these novel 16S rRNA gene sequences. BLAST and phylogeny analyses allowed for the identification of Nodosilinea and Oxynema strains, genera already known to exhibit poor morphological diacritic traits. In addition, several Nostoc and Leptolyngbya morphotypes of the mangrove strains may represent new generic entities, as they were distantly affiliated with true genera clades. The presence of non-ribosomal peptide synthetase, polyketide synthase, microcystin and saxitoxin genes were detected in 20.5%, 100%, 37.5% and 33.3%, respectively, of the 44 tested isolates. A total of 134 organic extracts obtained from 44 strains were tested against microorganisms, and 26% of the extracts showed some antimicrobial activity. This is the first polyphasic study of cultured cyanobacteria from Brazilian mangrove ecosystems using morphological, genetic and biological approaches. (C) 2014 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 06/01671-2 - Caracterização molecular de cianobactérias isoladas de manguezais do Estado de São Paulo e distribuição de cianopeptídeos
• Beneficiário: Caroline Souza Pamplona da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/18732-0 - Produtos naturais bioativos de cianobactérias marinhas
• Beneficiário: Marcelo Gomes Marçal Vieira Vaz
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/00321-3 - Diversidade e atividade funcional de cianobactérias do Arquipélago Shetlands do Sul, Continente Antártico
• Beneficiário: Diego Bonaldo Genuário
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático