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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Haemophilus influenzae porine ompP2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence bacterial capacity

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Autor(es):
Varela, Julia Nogueira [1] ; Kraehenbuehl Amstalden, Maria Cecilia [1] ; Carneiro Pereira, Rafaella Fabiana [1] ; de Hollanda, Luciana Maria [1] ; Ceragioli, Helder Jose [2] ; Baranauskas, Vitor [2] ; Lancellotti, Marcelo [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem, LABIOTEC Biotechnol Lab, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Semicond Instruments & Photon, NanoEng NanoEngn & Diamond Lab, Sch Elect & Comp E, BR-13083852 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF NANOBIOTECHNOLOGY; v. 12, APR 16 2014.
Citações Web of Science: 10
Resumo

Background: H. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup - aegyptius - as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor. This work studied the horizontal transference of the ompP2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased. Material and methods: In this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains. Results: The nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type. Conclusions: Graphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the ompP2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines. (AU)

Processo FAPESP: 12/15046-3 - Estudo do genoma e metaboloma de linhagens de Haemophilus influenzae biótipo aegyptius causadores da febre púrpura brasileira
Beneficiário:Marcelo Lancellotti
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/21685-6 - Mecanismos de aquisição de resistência a antibióticos por bactérias causadoras de infecção do trato urinário: uma abordagem farmacogenômica e biofarmacêutica
Beneficiário:Marcelo Lancellotti
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/14079-2 - Uso de nanopartículas em vacinas contra meningites bacterianas
Beneficiário:Marcelo Lancellotti
Linha de fomento: Auxílio à Pesquisa - Regular