Synthesis and characterization of xanthan-hydroxyapatite nanocomposites for cellular uptake

Autor(es)

Bueno, Vania Blasques ^[1] ; Bentini, Ricardo ^[1] ; Catalani, Luiz Henrique ^[1] ; Barbosa, Leandro R. S. ^[2] ; Siqueira Petri, Denise Freitas ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Fis, DFGE, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 37, p. 195-203, APR 1 2014.
• Citações Web of Science: 19

Resumo

In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp). XnHAp and XnHApSr were less crystalline than HAp, as revealed by X-ray diffraction. Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7 +/- 1) mV, determined for HAp, to -(17 +/- 3) mV and -(25 +/- 3) mV, respectively. This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140 +/- 10) nm long and (8 +/- 2) nm wide, as determined by scanning electron microscopy and atomic force microscopy. XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr). Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix. Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio. XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/13034-2 - Produção de suportes biodegradáveis de hidrogel de polissacarídeos para crescimento celular
• Beneficiário: Vânia Aparecida Blasques Bueno Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/21442-6 - Polímeros sintéticos e naturais aplicados à engenharia de tecido
• Beneficiário: Luiz Henrique Catalani
• Modalidade de apoio: Auxílio à Pesquisa - Temático