| Texto completo | |
| Autor(es): Mostrar menos - |
Castro, Lilian dos Santos
[1]
;
Pedersoli, Wellington Ramos
[1]
;
Antonio, Amanda Cristina Campos
[1]
;
Steindorff, Andrei Stecca
[2]
;
Silva-Rocha, Rafael
[1]
;
Martinez-Rossi, Nilce M.
[3]
;
Rossi, Antonio
[1]
;
Brown, Neil Andrew
[4, 5]
;
Goldman, Gustavo H.
[4, 5]
;
Faa, Vitor M.
[1]
;
Persinoti, Gabriela F.
[3]
;
Silva, Roberto Nascimento
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Brasilia, Dept Biol Celular, BR-70910900 Brasilia, DF - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[4] Univ Sao Francisco, Fac Ciencias Farmaceut Ribeirao Preto, Campinas - Brazil
[5] Lab Nacl Ciencia & Technol Bioetanol, Campinas - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOTECHNOLOGY FOR BIOFUELS; v. 7, MAR 21 2014. |
| Citações Web of Science: | 44 |
| Resumo | |
Background: The filamentous fungus Trichoderma reesei is a major producer of lignocellulolytic enzymes utilized by bioethanol industries. However, to achieve low cost second generation bioethanol production on an industrial scale an efficient mix of hydrolytic enzymes is required for the deconstruction of plant biomass. In this study, we investigated the molecular basis for lignocellulose-degrading enzyme production T. reesei during growth in cellulose, sophorose, and glucose. Results: We examined and compared the transcriptome and differential secretome (2D-DIGE) of T. reesei grown in cellulose, sophorose, or glucose as the sole carbon sources. By applying a stringent cut-off threshold 2,060 genes were identified as being differentially expressed in at least one of the respective carbon source comparisons. Hierarchical clustering of the differentially expressed genes identified three possible regulons, representing 123 genes controlled by cellulose, 154 genes controlled by sophorose and 402 genes controlled by glucose. Gene regulatory network analyses of the 692 genes differentially expressed between cellulose and sophorose, identified only 75 and 107 genes as being specific to growth in sophorose and cellulose, respectively. 2D-DIGE analyses identified 30 proteins exclusive to sophorose and 37 exclusive to cellulose. A correlation of 70.17% was obtained between transcription and secreted protein profiles. Conclusions: Our data revealed new players in cellulose degradation such as accessory proteins with non-catalytic functions secreted in different carbon sources, transporters, transcription factors, and CAZymes, that specifically respond in response to either cellulose or sophorose. (AU) | |
| Processo FAPESP: | 10/15683-8 - Estudos de sinalização celular e mecanismos de indução da formação de celulases pelo fungo Trichoderma reesei (Hypocrea jecorina) |
| Beneficiário: | Roberto do Nascimento Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores |