| Texto completo | |
| Autor(es): |
Gershlick, David C.
[1]
;
Lousa, Carine de Marcos
[1]
;
Foresti, Ombretta
[1]
;
Lee, Andrew J.
[1]
;
Pereira, Estela A.
[1]
;
dasilva, Luis L. P.
[1]
;
Bottanelli, Francesca
[1]
;
Denecke, Jurgen
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Leeds, Fac Biol Sci, Ctr Plant Sci, Sch Biol, Leeds LS2 9JT, W Yorkshire - England
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLANT CELL; v. 26, n. 3, p. 1308-1329, MAR 2014. |
| Citações Web of Science: | 19 |
| Resumo | |
The cycling of vacuolar sorting receptors (VSRs) between early and late secretory pathway compartments is regulated by signals in the cytosolic tail, but the exact pathway is controversial. Here, we show that receptor targeting in tobacco (Nicotiana tabacum) initially involves a canonical coat protein complex II-dependent endoplasmic reticulum-to-Golgi bulk flow route and that VSR-ligand interactions in the cis-Golgi play an important role in vacuolar sorting. We also show that a conserved Glu is required but not sufficient for rate-limiting YXX phi-mediated receptor trafficking. Protein-protein interaction studies show that the VSR tail interacts with the mu-subunits of plant or mammalian clathrin adaptor complex AP1 and plant AP4 but not that of plant and mammalian AP2. Mutants causing a detour of full-length receptors via the cell surface invariantly cause the secretion of VSR ligands. Therefore, we propose that cycling via the plasma membrane is unlikely to play a role in biosynthetic vacuolar sorting under normal physiological conditions and that the conserved Ile-Met motif is mainly used to recover mistargeted receptors. This occurs via a fundamentally different pathway from the prevacuolar compartment that does not mediate recycling. The role of clathrin and clathrin-independent pathways in vacuolar targeting is discussed. (AU) | |
| Processo FAPESP: | 09/50650-6 - Elucidação de mecanismos celulares e moleculares da modulação negativa de proteínas da superfície celular por NEF do HIV-1 |
| Beneficiário: | Luis Lamberti Pinto da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |