Formation of carbonated hydroxyapatite films on metallic surfaces using dihexadecyl phosphate-LB film as template

Autor(es)

de Souza, Israel D. ^[1] ; Cruz, Marcos A. E. ^[1] ; de Faria, Amanda N. ^{[1, 2]} ; Zancanela, Daniela C. ^[1] ; Simao, Ana M. S. ^[1] ; Ciancaglini, Pietro ^[1] ; Ramos, Ana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 118, p. 31-40, JUN 1 2014.
• Citações Web of Science: 18

Resumo

Hydroxyapatite serves as a bioactive material for biomedical purposes, because it shares similarities with the inorganic part of the bone. However, how this material deposits on metallic surfaces using biomimetic matrices remains unclear. In this study, we deposited dihexadecyl phosphate, a phospholipid that bears a simple chemical structure, on stainless steel and titanium surfaces using the Langmuir-Blodgett (LB) technique: we employed the resulting matrix to grow carbonated hydroxyapatite. We obtained the calcium phosphate coating via a two-step process: we immersed the surfaces modified with the LB films into phosphate buffer, and then, we exposed the metal to a solution that simulated the concentration of ions in the human plasma. The latter step generated carbonated hydroxyapatite, the same mineral existing in the bone. The free energy related to the surface roughness and composition increased after we modified the supports. We investigated the film morphology by scanning electron and atomic force microscopies and determined surface composition by infrared spectroscopy and energy dispersive X-ray. We also studied the role of the surface roughness and the surface chemistry on cell viability. The surface-modified Ti significantly increased osteoblastic cells proliferation, supporting the potential use of these surfaces as osteogenic materials. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/20946-3 - Estudo do crescimento de fosfatos e carbonatos de cálcio sobre matrizes orgânicas depositadas sobre suportes metálicos: obtenção de biomateriais osteoindutores
• Beneficiário: Ana Paula Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular