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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Microsatellite Organization in the Grasshopper Abracris flavolineata (Orthoptera: Acrididae) Revealed by FISH Mapping: Remarkable Spreading in the A and B Chromosomes

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Autor(es):
Milani, Diogo [1] ; Cabral-de-Mello, Diogo Cavalcanti [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Inst Biociencias IB, Dept Biol, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 9, n. 5 MAY 28 2014.
Citações Web of Science: 17
Resumo

With the aim of acquiring deeper knowledge about repetitive DNAs chromosomal organization in grasshoppers, we used fluorescent in situ hybridization (FISH) to map the distribution of 16 microsatellite repeats, including mono-, di-, tri- and tetra-nucleotides, in the chromosomes of the species Abracris flavolineata (Acrididae), which harbors B chromosome. FISH revealed two main patterns: (i) exclusively scattered signals, and (ii) scattered and specific signals, forming evident blocks. The enrichment was observed in both euchromatic and heterochromatic areas and only the motif (C)(30) was absent in heterochromatin. The A and B chromosomes were enriched with all the elements that were mapped, being observed in the B chromosome more distinctive blocks for (GA)(15) and (GAG)(10). For A complement distinctive blocks were noticed for (A)(30), (CA)(15), (CG)(15), (GA)(15), (CAC)(10), (CAA)(10), (CGG)(10), (GAA)(10), (GAC)(10) and (GATA)(8). These results revealed an intense spreading of microsatellites in the A. flavolineata genome that was independent of the A+T or G+C enrichment in the repeats. The data indicate that the microsatellites compose the B chromosome and could be involved in the evolution of this element in this species, although no specific relationship with any A chromosome was observed to discuss about its origin. The systematic analysis presented here contributes to the knowledge of repetitive DNA chromosomal organization among grasshoppers including the B chromosomes. (AU)

Processo FAPESP: 11/19481-3 - Contribuições a elucidação do significado biológico de cromossomos B utilizando como modelos Rhammatocerus brasiliensis (Orthoptera) e Haplochromis obliquidens (Teleostei): estrutura, evolução e padrões de modificações em proteínas histônicas
Beneficiário:Diogo Cavalcanti Cabral de Mello
Linha de fomento: Auxílio à Pesquisa - Regular