Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans

Autor(es)

Freire, Fernanda ^[1] ; Borges Pereira Costa, Anna Carolina ^[1] ; Pereira, Cristiane Aparecida ^[1] ; Beltrame Junior, Milton ^[2] ; Junqueira, Juliana Campos ^[1] ; Cardoso Jorge, Antonio Olavo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, BR-12245000 Sao Paulo - Brazil

^[2] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, BR-12244000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 29, n. 3, p. 949-955, MAY 2014.
• Citações Web of Science: 24

Resumo

Candida albicans is an opportunistic yeast that can cause oral candidosis through the formation of a biofilm, an important virulence factor that compromises the action of antifungal agents. The objective of this study was to compare the effect of rose bengal (RB)- and eosin Y (EY)-mediated photodynamic inactivation (PDI) using a green light-emitting diode (LED; 532 +/- 10 nm) on planktonic cells and biofilms of C. albicans (ATCC 18804). Planktonic cultures were treated with photosensitizers at concentrations ranging from 0.78 to 400 mu M, and biofilms were treated with 200 mu M of photosensitizers. The number of colony-forming unit per milliliter (CFU/mL) was compared by analysis of variance and Tukey's test (P a parts per thousand currency signaEuro parts per thousand 0.05). After treatment, one biofilm specimen of the control and PDI groups were examined by scanning electron microscopy. The photosensitizers (6.25, 25, 50, 200, and 400 mu M of EY, and 6.25 mu M of RB or higher) significantly reduced the number of CFU/mL in the PDI groups when compared to the control group. With respect to biofilm formation, RB- and EY-mediated PDI promoted reductions of 0.22 log(10) and 0.45 log(10), respectively. Scanning electron microscopy showed that the two photosensitizers reduced fungal structures. In conclusion, EY- and RB-mediated PDI using LED irradiation significantly reduced C. albicans planktonic cells and biofilms. (AU)

• Processo FAPESP: 10/00879-4 - MICRORGANISMOS OPORTUNISTAS EM LESÕES DE ESTOMATITE PROTÉTICA E INFLUÊNCIA DA TERAPIA FOTODINÂMICA NOS FATORES DE VIRULÊNCIA DE Candida spp.
• Beneficiário: Cristiane Aparecida Pereira Correia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21346-7 - GENES DE Candida albicans COM FUNÇÃO DESCONHECIDA QUANTO À FORMAÇÃO DE BIOFILME, CARACTERÍSTICAS BIOLÓGICAS E INTERAÇÃO PATÓGENO-HOSPEDEIRO
• Beneficiário: Anna Carolina Borges Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/52048-1 - Efeitos da terapia fotodinâmica e de óleos essenciais de Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW e Curcuma zedoaria Roscoe, in vitro, em biofilmes formados com Candida albicans, Staphylococcus aureus e Streptococcus mutans
• Beneficiário: Antonio Olavo Cardoso Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/12115-9 - COMPARAÇÃO ENTRE OS FOTOSSENSIBILIZADORES ROSA BENGALA E EOSINA Y, NA TERAPIA FOTODINÂMICA in vitro EM CULTURAS PLANCTÔNICAS E BIOFILMES DE Candida albicans.
• Beneficiário: Fernanda Freire
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica