Busca avançada
Ano de início
Entree
Conteúdo relacionado
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

PRODUCTION OF NATIVE FLAGELLIN FROM Salmonella Typhimurium IN A BIOREACTOR AND PURIFICATION BY TANGENTIAL ULTRAFILTRATION

Texto completo
Autor(es):
Oliveira, B. H. [1] ; Silva, M. R. [2] ; Braga, C. J. M. [3] ; Massis, L. M. [3] ; Ferreira, L. C. S. [3] ; Sbrogio-Almeida, M. E. [2] ; Takagi, M. [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Parana, Dept Engn Quim, BR-80060000 Curitiba, PR - Brazil
[2] Inst Butantan, Lab Fermentacao, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Chemical Engineering; v. 28, n. 4, p. 575-584, OCT-DEC 2011.
Citações Web of Science: 5
Resumo

Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up. (AU)

Processo FAPESP: 06/52064-9 - Flagelinas de salmonella como adjuvantes vacinais administrados por vias de mucosa e parenteral.
Beneficiário:Maria Elisabete Sbrogio-Almeida
Modalidade de apoio: Auxílio à Pesquisa - Regular