| Full text | |
| Author(s): |
Oliveira, B. H.
[1]
;
Silva, M. R.
[2]
;
Braga, C. J. M.
[3]
;
Massis, L. M.
[3]
;
Ferreira, L. C. S.
[3]
;
Sbrogio-Almeida, M. E.
[2]
;
Takagi, M.
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Parana, Dept Engn Quim, BR-80060000 Curitiba, PR - Brazil
[2] Inst Butantan, Lab Fermentacao, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Brazilian Journal of Chemical Engineering; v. 28, n. 4, p. 575-584, OCT-DEC 2011. |
| Web of Science Citations: | 5 |
| Abstract | |
Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production process of purified native FliC flagellin of Salmonella Typhimurium. The culture conditions in shakers were established with medium devoid of animal-derived components. In bioreactors, culture conditions were established in order to obtain flagellin from the culture supernatant by tangential ultrafiltration (TUF). The concentrated 750 kDa cut-off TUF fraction had a purification factor of 1.5 and a recovery yield of 52.2% for flagellin. The volumetric production of flagellin using the described procedure achieved around 307 mg/L of culture, which represented a significant improvement over previously reported methods. These results permit the development of production and purification processes that can be easily scaled up. (AU) | |