Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Simultaneous evaluation of in vivo glucocorticoid sensitivity and expression of glucocorticoid receptor alpha-isoform in rheumatoid arthritis patients

Texto completo
Autor(es):
Cobra, Jayme F. [1] ; Melo, Murilo R. [2] ; Faria, Claudia D. C. [2] ; Longui, Carlos Alberto [2] ; Monte, Osmar [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Fac Med Sci, Rheumatol Discipline, Sao Paulo, SP - Brazil
[2] Fac Med Sci, Dept Physiol, Mol Med Lab, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Arquivos Brasileiros de Endocrinologia e Metabologia; v. 53, n. 1, p. 24-30, FEB 2009.
Citações Web of Science: 6
Resumo

Objectives: To analyze glucocorticoid (GC) sensitivity using intravenous very low dose dexamethasone suppression test (IV-VLD-DST) in patients with rheumatoid arthritis (RA) and its correlation with glucocorticoid receptor alpha-isoform (GR alpha) gene expression. Methods: We evaluated 20 healthy controls and 32 RA patients with Health Assessment Questionnaire (HAQ) and Disease Activity Score 28 joints (DAS) scores and IV-VLD-DST and GR alpha expression in mononuclear cells. Results: Basal cortisol and the percentage of cortisol reduction after IV-VLD-DST were lower in RA patients than in controls, whereas GR alpha expression was similar among groups. In the RA group there was an inverse correlation between GR alpha expression and the percentage of cortisol suppression that was not observed in controls. There was a direct relationship between DAS and GR alpha expression. Conclusions: Mechanisms involved in GC resistance observed in patients with RA are possibly not at the level of GR alpha gene expression, since it was similar among groups and GR alpha increased with disease activity. Arq Bras Endocrinol Metab. 2009;53(1):24-30. (AU)

Processo FAPESP: 04/03208-2 - Quantificação da expressão de receptores glicocorticóides por PCR em tempo real e sua correlação com a sensibilidade individual aos glicocorticóides
Beneficiário:Carlos Alberto Longui
Linha de fomento: Auxílio à Pesquisa - Regular