Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Preparation, characterization, and in vitro phototoxic effect of zinc phthalocyanine cucurbit[7]uril complex encapsulated into liposomes

Texto completo
Autor(es):
Bolfarini, Gisele C. [1] ; Siqueira-Moura, Marigilson P. [2] ; Demets, Gregoire J. F. [3] ; Tedesco, Antonio C. [4]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
[2] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
[3] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
[4] Univ Sao Paulo. Fac Filosofia Ciencias & Letras Ribeirao Preto
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: DYES AND PIGMENTS; v. 100, p. 162-167, JAN 2014.
Citações Web of Science: 15
Resumo

Inclusion complexes improve the solubility, dissolution, and bioavailability of hydrophobic drugs. In this study, we used the cucurbit{[}7]uril (CB{[}7]) to improve the solubility of zinc phthalocyanine (ZnPc) in water. The CB{[}7]:ZnPc complex was prepared by co-evaporation, characterized it by H-1 NMR spectroscopy, differential scanning calorimetry, X-ray diffraction, UV-vis spectroscopy, and molecular simulation. Next, the CB{[}7]:ZnPc inclusion complex was incorporated into liposomes (CB{[}7]:ZnPc-Lp) to obtain a drug delivery system and assess the in vitro phototoxicity of both free and liposomal CB{[}7]:ZnPc on B16-F10 melanoma cells. H-1 NMR confirmed that the CB{[}7]/ZnPc proportion in the inclusion complex was 1:1, as corroborated by X-ray diffraction, differential scanning calorimetry, and UV-vis spectroscopy. Molecular simulation evidenced that the structures of ZnPc and CB{[}7] interact in the inclusion complex. The thin lipid film method with subsequent extrusion furnished liposomes containing CB{[}7]:ZnPc compared with the free inclusion complex, CB{[}7]:ZnPc-Lp exerted excellent phototoxic effect on melanoma cells. In conclusion, inclusion complexes effectively solubilize hydrophobic compounds, such as zinc phthalocyanine and allow for their incorporation into liposomes, generating excellent drug delivery systems. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
Beneficiário:Antonio Claudio Tedesco
Linha de fomento: Auxílio à Pesquisa - Temático