Regulation of visfatin by microbial and biomechanical signals in PDL cells

Autor(es)

Boas Nogueira, Andressa Vilas ^[1] ; Nokhbehsaim, Marjan ^[2] ; Eick, Sigrun ^[3] ; Bourauel, Christoph ^[4] ; Jaeger, Andreas ^[5] ; Jepsen, Soren ^[6] ; Cirelli, Joni Augusto ^[7] ; Deschner, James ^[8]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP. Dept Diag & Surg

^[2] Univ Bonn. Ctr Dentomaxillofacial Med

^[3] Univ Bern. Dept Periodontol

^[4] Univ Bonn. Ctr Dentomaxillofacial Med

^[5] Univ Bonn. Clin Res Unit 208

^[6] Univ Bonn. Clin Res Unit 208

^[7] Univ Estadual Paulista UNESP. Dept Diag & Surg

^[8] Univ Bonn. Ctr Dentomaxillofacial Med

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 18, n. 1, p. 171-178, JAN 2014.
• Citações Web of Science: 25

Resumo

This in vitro study was established to examine whether visfatin thought to be a link between periodontitis and obesity is produced by periodontal ligament (PDL) cells and, if so, whether its synthesis is modulated by microbial and/or biomechanical signals. PDL cells seeded on BioFlexA (R) plates were exposed to the oral pathogen Fusobacterium nucleatum ATCC 25586 and/or subjected to biomechanical strain for up to 3 days. Gene expression of visfatin and toll-like receptors (TLR) 2 and 4 was analyzed by RT-PCR, visfatin protein synthesis by ELISA and immunocytochemistry, and NF kappa B nuclear translocation by immunofluorescence. F. nucleatum upregulated the visfatin expression in a dose- and time-dependent fashion. Preincubation with neutralizing antibodies against TLR2 and TLR4 caused a significant inhibition of the F. nucleatum-upregulated visfatin expression at 1 day. F. nucleatum stimulated the NF kappa B nuclear translocation. Biomechanical loading reduced the stimulatory effects of F. nucleatum on visfatin expression at 1 and 3 days and also abrogated the F. nucleatum-induced NF kappa B nuclear translocation at 60 min. Biomechanical loading inhibited significantly the expression of TLR2 and TLR4 at 3 days. The regulatory effects of F. nucleatum and/or biomechanical loading on visfatin expression were also observed at protein level. PDL cells produce visfatin, and this production is enhanced by F. nucleatum. Biomechanical loading seems to be protective against the effects of F. nucleatum on visfatin expression. Visfatin produced by periodontal tissues could play a major role in the pathogenesis of periodontitis and the interactions with obesity and other systemic diseases. (AU)

• Processo FAPESP: 10/07771-4 - Efeito da movimentação ortodôntica na progressão da doença periodontal. Estudos in vivo e in vitro.
• Beneficiário: Andressa Vilas Boas Nogueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/13752-5 - Contribuição das forças biomecânicas na destruição periodontal: estudos in vitro e in vivo
• Beneficiário: Joni Augusto Cirelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular