Synergistic action of co-expressed xylanase/laccase mixtures against milled sugar cane bagasse

Autor(es):Mostrar menos -

Fonseca-Maldonado, Raquel ^[1] ; Ribeiro, Lucas F. ^[1] ; Furtado, Gilvan P. ^[1] ; Arruda, Leticia M. ^[1] ; Meleiro, Luana P. ^[2] ; Alponti, Juliana S. ^[2] ; Botelho-Machado, Carla ^{[2, 3]} ; Vieira, Davi S. ^[4] ; Bonneil, Eric ^[5] ; Melo Furriel, Rosa dos Prazeres ^[2] ; Thibault, Pierre ^[5] ; Ward, Richard J. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim & Imunol, FMRP, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim, FFCLRP, Ribeirao Preto, SP - Brazil

^[3] CNPEM, CTBE, Lab Nacl Ciencia & Tecnol Bioetanol, Sao Paulo - Brazil

^[4] Univ Fed Rio Grande do Norte Natal, Inst Quim, Natal, RN - Brazil

^[5] Univ Montreal, Inst Res Immunol & Canc, Montreal, PQ H3C 3J7 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 49, n. 7, p. 1152-1161, JUL 2014.
• Citações Web of Science: 9

Resumo

The primary plant cell wall is composed of cellulose, hemicellulose, lignin and protein in a stable matrix. The concomitant depolymerization of lignin by laccase and of hemicelluloses by xylanase can improve lignocellulose degradation in the production of second generation biofuels. A thermophilic variant of xylanase A (XynAG3) and the thermostable laccase (CotA), both from Bacillus subtilis, were produced in co-transformed Pichia pastoris strain GS115. Mobility changes in SDS-PAGE after Endo H digestion indicat63 that both enzymes were glycosylated. The maximum catalytic activity of the XynAG3(Pp) and the CotA(Pp) was observed at 58 degrees C and 75 degrees C, respectively, and both enzymes presented high activity at pH 5.0. The half-life at 60 degrees C of XynAG3(Pp) and CotA(Pp) was 150 min and 540 min, respectively. The relative levels of CotA(Pp) and XynAG3(Pp) in culture broths were altered by the concentration of methanol used for induction, and CotA(Pp):XynAG3(Pp) ratios of 1:1.5 and 1:2 were evaluated against milled sugar-cane bagasse. The highest activity was observed at a 1:2 ratio of CotA(Pp):XynAG3(Pp), and was 44% higher as compared to the sum of the activities of the individual enzymes in the same assay conditions. These results demonstrate the synergistic action between an endoxylanase and a laccase against the natural lignocellulosic substrate. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/18850-2 - Identificação, caracterização e engenharia de enzimas que degradam a parede celular das plantas
• Beneficiário: Richard John Ward
• Modalidade de apoio: Auxílio à Pesquisa - Temático
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• Modalidade de apoio: Bolsas no Brasil - Doutorado
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• Modalidade de apoio: Bolsas no Brasil - Mestrado
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado