Genotyping of Yersinia enterocolitica biotype 1A strains from clinical and nonclinical origins by pulsed-field gel electrophoresis

Autor(es)

Campioni, Fabio ^[1] ; Falcao, Juliana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Brazilian Reference Ctr Yersinia Spp Other Y Pest, BR-14040903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Canadian Journal of Microbiology; v. 60, n. 6, p. 419-424, JUN 2014.
• Citações Web of Science: 4

Resumo

Yersinia enterocolitica biotype 1A (B1A) strains are considered mainly nonpathogenic. However, some studies considered strains of this biotype to be the causal agents of infections in humans and animals. In South America, there are no studies that have compared clinical and nonclinical strains of B1A typed by pulsed-field gel electrophoresis (PFGE) and none that have compared the capability of different enzymes on typing these strains. This study typed 51 Y. enterocolitica B1A strains isolated in Brazil and Chile by PFGE, testing the enzymes XbaI, NotI, and XhoI. The resulting dendrograms discriminated the strains in 47, 40, and 49 pulsotypes generated by the cleavage with the enzymes XbaI, NotI, and XhoI, respectively. The majority of the strains were grouped independently of their clinical or nonclinical origins. The high discriminatory power of PFGE confirmed the heterogeneity of B1A strains but could not divide the strains studied into clusters that differed in the frequency of some virulence genes as observed in studies using other methodologies. (AU)

• Processo FAPESP: 06/51434-7 - Rastreamento epidemiológico de amostras de Yersinia enterocolitica biotipo 1A de origens diversas e classificação de amostras de Yersinia sp. que não se enquadram bioquimicamente nas espécies conhecidas, com o auxílio técnicas fenotípicas e moleculares
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular