Fertility and uterine hemodynamic in cows after artificial insemination with semen assessed by fluorescent probes

Autor(es)

Oliveira, Bruna Marcele ^[1] ; Arruda, Rubens Paes ^[1] ; Thome, Helder Esteves ^[1] ; Maturana Filho, Milton ^[2] ; Oliveira, Guilherme ^[1] ; Guimaraes, Carina ^[2] ; Nichi, Marcilio ^[2] ; Silva, Luciano Andrade ^[3] ; Carvalho Celeghini, Eneiva Carla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Vet Med & Anim Sci FMVZ, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Ctr Biotechnol Anim, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Coll Vet Med & Anim Sci FMVZ, Dept Anim Reprod, Sao Paulo - Brazil

^[3] Univ Sao Paulo, FZEA, Dept Vet Med, Lab Theriogenol Dr OJ Ginther, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 82, n. 5, p. 767-772, SEP 15 2014.
• Citações Web of Science: 7

Resumo

Fluorescent probes (propidium iodide, Hoechst 33342, fluorescein isothiocyanate-conjugated Pisum sativum agglutinin, and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide) were used to simultaneously evaluate the integrity of plasma and acrosomal membranes as well as mitochondrial membrane function in cryopreserved bovine semen and to verify its influence on fertility and postinsemination uterine vascularization. One hundred eighty-two Nellore cows were distributed for artificial insemination (AI) using semen batches separated according to the cell percentage presenting intact plasma membrane, intact acrosome, and high mitochondrial function (IPIAH): group G (44.5% IPIAH, n = 68), group M (23.0% IPIAH, n = 56), and group R (8.5% IPIAH, n = 58). The uterine hemodynamic was evaluated by Doppler sonogram in three periods: 30 hours before Al, 4 and 24 hours after Al were considered the resistance index and the uterine vascularization score. The pregnancy rate of group G (64.7%) was greater (P > 0.05) compared with group R (36.2%), but both did not differ from group M (50.0%). There was no effect (P > 0.05) of semen quality on uterine vascularization. Greater vascularization was noticed 4 hours after Al than 30 hours before and 24 hours after Al. Semen evaluation using fluorescent probes contributes to predicting fertilizing potential of semen. The use of semen with less percentage of IPIAH sperm does not alter uterine hemodynamic in cows. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/03997-8 - Taxa de fertilidade e resposta inflamatória uterina avaliada por ultrassonografia com Doppler colorido após inseminação artificial com sêmen de diferentes qualidades espermáticas avaliadas por associações de sondas fluorescentes em bovinos
• Beneficiário: Bruna Marcele Martins de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/50365-0 - Fertilidade e resposta inflamatória uterina em bovinos após inseminação artificial com sêmen avaliado por associações de sondas fluorescentes
• Beneficiário: Eneiva Carla Carvalho Celeghini
• Modalidade de apoio: Auxílio à Pesquisa - Regular