O envolvimento dos macrófagos nos efeitos da suplementação com ácidos graxos ômega-3 sobre a cicatrização de feridas: da bancada para o leito do paciente

• Processo: 18/11037-6
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de novembro de 2018
• Data de Término da vigência: 31 de outubro de 2020
• Área do conhecimento: Ciências Biológicas - Fisiologia - Fisiologia Geral
• Acordo de Cooperação: University of Surrey
• Pesquisador responsável: Hosana Gomes Rodrigues
• Beneficiário: Hosana Gomes Rodrigues
• Pesquisador Responsável no exterior: Fernando Oneissi Martinez Estrada
• Instituição Parceira no exterior: University of Surrey, Inglaterra
• Instituição Sede: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brasil
• Auxílio(s) vinculado(s): 19/24998-7 - Ácido garoa docosahexaenóico diminui a resolução da inflamação e prejudica a qualidade do tecido cutâneo cicatrizado, PUB.ART; 19/16193-9 - Biossíntese de globosídeos durante o reparo da pele, AP.R SPRINT
• Assunto(s): Nutrição Fisiologia da pele Inflamação Lipídeos
• Palavra(s)-Chave do Pesquisador: Inflamação | lipídios | Nutrição | Reparo Tecidual | Fisiologia da pele

Resumo

Defective wound healing is a common complication of ageing, diabetes and obesity and a strong relationship between malnutrition, poor wound healing and infection exists. Omega-3 fatty acids are fish oil derivatives associated with maintenance of optimum immune responses, since it activate cells of the innate immune system called macrophages. Macrophages orchestrate wound healing by fighting invading pathogens; recruiting endothelial cells and fibroblasts to damaged areas for tissue repair, and eliminating dying cells and debris. Despite strong experimental evidence behind omega-3 benefits, there is no formal demonstration that peri-operative (when the nutritional support initiates before surgery and lasts days or months after it) omega-3 supplementation improves recovery. The aim of this multi-species project is to determine if peri-operative omega-3 fatty acids improve wound healing and prevent post-operative infection. The human study includes a clinical trial sponsored by Royal Surrey County Hospital NHS Foundation Trust where surgery patients will receive peri-operative omega-3 supplements. Blood cells, serum and peritoneal cells will be collected and characterised using cytokine measurement, CYTOF, ImageStream analysis, and whole cell lipidomics. In mice, bone marrow transplantation and macrophage depletion will clarify how omega-3 affect specifically macrophage functions. Parallel human/murine organoid assays will corroborate the role of omega- 3 on macrophages functions during wound healing in alignment with NC3Rs priorities. We expect to clarify the mechanisms involved in the peri-operative omega- 3 fatty acid effects on tissue repair and will have direct repercussion on patient management worldwide. (AU)

Publicações científicas

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

BURGER, BEATRIZ; KUHL, CAROLINA M. C.; CANDREVA, THAMIRIS; CARDOSO, RENATO DA S.; SILVA, JESSICA R.; CASTELUCCI, BIANCA G.; CONSONNI, SILVIO R.; FISK, HELENA L.; CALDER, PHILIP C.; VINOLO, MARCO AURELIO R.; et al. Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice. SCIENTIFIC REPORTS, v. 9, JUN 24 2019. (13/06810-4, 18/11037-6, 14/15127-9)

BURGER, BEATRIZ; RODRIGUES, HOSANA GOMES; GUEST, PC. Cutaneous Manifestations of COVID-19: Early Diagnosis and Prognostic Information. IDENTIFCATION OF BIOMARKERS, NEW TREATMENTS, AND VACCINES FOR COVID-19, v. 1327, p. 9-pg., 2021-01-01. (19/23140-9, 18/11037-6)