Study of the effects of fatty acids of varying chain length on carnitine palmitoyltransferase i and ii activity in human tumour cell lines.

• Processo: 95/06996-0
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de dezembro de 1995
• Data de Término da vigência: 31 de maio de 1997
• Área do conhecimento: Ciências Biológicas - Bioquímica - Metabolismo e Bioenergética
• Pesquisador responsável: Alison Colquhoun
• Beneficiário: Alison Colquhoun
• Instituição Sede: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Assunto(s): Ácidos graxos Metabolismo dos lipídeos
• Palavra(s)-Chave do Pesquisador: Carnitine Palmitoyltransferase | Fatty Acids | Human Tumour Cells | Lipid Metabolism

Resumo

Carnitine palmitoyltransferase (CPT) I and II are the enzymes which control the rate of entry of fatty acyl CoA into the mitochondrion, thereby controlling the rate of subsequent beta-oxidation. These enzymes are able to utilize several fatty acids and in both liver and heart their activity is dependent upon the type of substrate available. The project will study the effects of fatty acids of varying chain length on the activity of CPT I and II in human tumour cells. In addition, the effects of antioxidant and prostaglandins will be studied in order to determine the possible involvement of either lipid peroxides or eicosanoids in the regulation of CPT activity and consequently the fatty acid oxidative capacity of human tumour cells. (AU)