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Characterization of Human Immunodeficiency Virus type 1 (HIV-1) in a cohort of recently infected persons from the State of São Paulo by full genome sequencing

Processo: 06/50096-0
Linha de fomento:Bolsas no Brasil - Pós-Doutorado
Vigência (Início): 01 de junho de 2006
Vigência (Término): 31 de janeiro de 2010
Área do conhecimento:Ciências da Saúde - Medicina - Clínica Médica
Pesquisador responsável:Ricardo Sobhie Diaz
Beneficiário:Sabri Saeed Mohamed Ahmed Al-Sanabani
Instituição-sede: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brasil
Vinculado ao auxílio:04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP, AP.TEM

Resumo

The tremendous genetic variability of HIV has a direct impact on the development of molecular diagnostic tests and is a serious hurdle that confronts research in the development of a vaccine to counter the virus. It is therefore important to monitor the distribution and dynamics of HIV subtypes to avoid vaccination failure and a pitfall for molecular diagnostics The main objective of the current study is to characterize HIV-1 virtually full-length genome in a well-characterized cohort of individuals with recently acquired HIV-1 infection from the state of Sao Paulo. A total of EDTA-blood from 150 individuals with recently acquired HIV-1 infection on the bases of criteria established by other study will be enrolled. Amplification of proviral DNA from PBMC will be carried out by nested PCR according to the protocol established in our laboratory and supposes to result in 5 overlapping DNA fragments covering nearly full-length HIV-1 genome. Products will be sequenced and each sequence will be processed through phylogenetic analysis. Information on sequence alignment, evolution, cohort statistics, host genetic background, epitope mapping, clinical, immunological, and epidemiological data will be cross-referenced to viral sequences to allow for subsequent association studies (AU)

Publicações científicas (13)
(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)
SANTANA, VINICIUS CANATO; ALMEIDA, RAFAEL RIBEIRO; RIBEIRO, SUSAN PEREIRA; DE SOUZA FERREIRA, LIUS CARLOS; KALIL, JORGE; ROSA, DANIELA SANTORO; CUNHA-NETO, EDECIO. Co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating factor increases human immunodeficiency virus-1 DNA vaccine-induced polyfunctional CD4(+) T-cell responses. Memórias do Instituto Oswaldo Cruz, v. 110, n. 8, p. 1010-1016, DEC 2015. Citações Web of Science: 6.
ALMEIDA, RAFAEL RIBEIRO; RAPOSO, RUI ANDRE SARAIVA; COIRADA, FERNANDA CAROLINE; DA SILVA, JAMILE RAMOS; DE SOUZA FERREIRA, LUIS CARLOS; KALIL, JORGE; NIXON, DOUGLAS F.; CUNHA-NETO, EDECIO. Modulating APOBEC expression enhances DNA vaccine immunogenicity. Immunology and Cell Biology, v. 93, n. 10, p. 868-876, NOV 2015. Citações Web of Science: 3.
VINICIUS CANATO SANTANA; RAFAEL RIBEIRO ALMEIDA; SUSAN PEREIRA RIBEIRO; LUÍS CARLOS DE SOUZA FERREIRA; JORGE KALIL; DANIELA SANTORO ROSA; EDECIO CUNHA NETO. Co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating factor increases human immunodeficiency virus-1 DNA vaccine-induced polyfunctional CD4+ T-cell responses. Memórias do Instituto Oswaldo Cruz, v. 110, n. 8, p. -, Dez. 2015.
SANABANI, SABRI SAEED; PESSOA, RODRIGO; SOARES DE OLIVEIRA, ANA CAROLINA; MARTINEZ, VANESSA POUZA; GIRET, MARIA TERESA MAIDANA; DE MENEZES SUCCI, REGINA CELIA; CARVALHO, KARINA; TOMIYAMA, CLAUDIA SATIKO; NIXON, DOUGLAS F.; SABINO, ESTER CERDEIRA; KALLAS, ESPER GEORGES. Variability of HIV-1 Genomes among Children and Adolescents from Sao Paulo, Brazil. PLoS One, v. 8, n. 5 MAY 7 2013. Citações Web of Science: 14.
MAHNKE, YOLANDA D.; SONG, KAIMEI; SAUER, MARIANA M.; NASON, MARTHA C.; GIRET, MARIA TERESA M.; CARVALHO, KARINA I.; COSTA, PRISCILLA R.; ROEDERER, MARIO; KALLAS, ESPER G. Early immunologic and virologic predictors of clinical HIV-1 disease progression. AIDS, v. 27, n. 5, p. 697-706, MAR 13 2013. Citações Web of Science: 7.
ALMEIDA, RAFAEL RIBEIRO; ROSA, DANIELA SANTORO; RIBEIRO, SUSAN PEREIRA; SANTANA, VINICIUS CANATO; KALLAS, ESPER GEORGES; SIDNEY, JOHN; SETTE, ALESSANDRO; KALIL, JORGE; CUNHA-NETO, EDECIO. Broad and Cross-Clade CD4(+) T-Cell Responses Elicited by a DNA Vaccine Encoding Highly Conserved and Promiscuous HIV-1 M-Group Consensus Peptides. PLoS One, v. 7, n. 9 SEP 18 2012. Citações Web of Science: 19.
DUDLEY, DAWN M.; CHIN, EMILY N.; BIMBER, BENJAMIN N.; SANABANI, SABRI S.; TAROSSO, LEANDRO F.; COSTA, PRISCILLA R.; SAUER, MARIANA M.; KALLAS, ESPER G.; O'CONNOR, DAVID H. Low-Cost Ultra-Wide Genotyping Using Roche/454 Pyrosequencing for Surveillance of HIV Drug Resistance. PLoS One, v. 7, n. 5 MAY 4 2012. Citações Web of Science: 59.
SANABANI, SABRI SAEED; DE SOUZA PASTENA, EVELYN REGINA; DA COSTA, ANTONIO CHARLYS; MARTINEZ, VANESSA POUZA; KLEINE-NETO, WALTER; SOARES DE OLIVEIRA, ANA CAROLINA; SAUER, MARIANA MELILLO; BASSICHETTO, KATIA CRISTINA; SANTOS OLIVEIRA, SOLANGE MARIA; IWASHITA TOMIYAMA, HELENA TOMOKO; SABINO, ESTER CERDEIRA; KALLAS, ESPER GEORGES. Characterization of Partial and Near Full-Length Genomes of HIV-1 Strains Sampled from Recently Infected Individuals in Sao Paulo, Brazil. PLoS One, v. 6, n. 10 OCT 14 2011. Citações Web of Science: 26.
SANABANI‚ S.S.; DE SOUZA PASTENA‚ É.R.; DA COSTA‚ A.C.; MARTINEZ‚ V.P.; KLEINE-NETO‚ W.; DE OLIVEIRA‚ A.C.S.; SAUER‚ M.M.; BASSICHETTO‚ K.C.; OLIVEIRA‚ S.M.S.; TOMIYAMA‚ H.T.I.; OTHERS. Characterization of partial and near full-length genomes of HIV-1 strains sampled from recently infected individuals in Sao Paulo‚ Brazil. PLoS One, v. 6, n. 10, p. e25869, 2011.
SANABANI, SABRI SAEED; DE SOUZA PASTENA, EVELYN REGINA; KLEINE NETO, WALTER; MARTINEZ, VANESSA POUZA; SABINO, ESTER CERDEIRA. Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in Sao Paulo, Brazil. VIROLOGY JOURNAL, v. 7, APR 16 2010. Citações Web of Science: 18.
SANABANI‚ S.S.; PASTENA‚ ER; NETO‚ W.K.; MARTINEZ‚ V.P.; SABINO‚ E.C.; OTHERS. Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in Sao Paulo‚ Brazil. VIROLOGY JOURNAL, v. 7, p. 74, 2010.
SANABANI, SABRI SAEED; DE SOUZA PASTENA, EVELYN REGINA; KLEINE NETO, WALTER; BARRETO, CLAUDIA C.; FERRARI, KELLY T.; KALMAR, ERIKA M. N.; FERREIRA, SUZETE; SABINO, ESTER CERDEIRA. Near full-length genome analysis of low prevalent human immunodeficiency virus type 1 subclade F1 in Sao Paulo, Brazil. VIROLOGY JOURNAL, v. 6, JUN 16 2009. Citações Web of Science: 9.
SANABANI‚ S.S.; PASTENA‚ ER; KLEINE NETO‚ W.; BARRETO‚ C.C.; FERRARI‚ K.T.; KALMAR‚ EM; FERREIRA‚ S.; SABINO‚ E.C.; OTHERS. Near full-length genome analysis of low prevalent human immunodeficiency virus type 1 subclade F1 in São Paulo‚ Brazil. VIROLOGY JOURNAL, v. 6, p. 78, 2009.

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