| Processo: | 06/50096-0 |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Data de Início da vigência: | 01 de junho de 2006 |
| Data de Término da vigência: | 31 de janeiro de 2010 |
| Área de conhecimento: | Ciências da Saúde - Medicina - Clínica Médica |
| Pesquisador responsável: | Ricardo Sobhie Diaz |
| Beneficiário: | Sabri Saeed Mohammed Ahmed Al-Sanabani |
| Instituição Sede: | Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brasil |
| Vinculado ao auxílio: | 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP, AP.TEM |
| Palavra(s)-Chave do Pesquisador: | Full Length Genome | Hiv-1 | Recenty Infected | Recombination | Sao Paulo | Subytpes |
Resumo The tremendous genetic variability of HIV has a direct impact on the development of molecular diagnostic tests and is a serious hurdle that confronts research in the development of a vaccine to counter the virus. It is therefore important to monitor the distribution and dynamics of HIV subtypes to avoid vaccination failure and a pitfall for molecular diagnostics The main objective of the current study is to characterize HIV-1 virtually full-length genome in a well-characterized cohort of individuals with recently acquired HIV-1 infection from the state of Sao Paulo. A total of EDTA-blood from 150 individuals with recently acquired HIV-1 infection on the bases of criteria established by other study will be enrolled. Amplification of proviral DNA from PBMC will be carried out by nested PCR according to the protocol established in our laboratory and supposes to result in 5 overlapping DNA fragments covering nearly full-length HIV-1 genome. Products will be sequenced and each sequence will be processed through phylogenetic analysis. Information on sequence alignment, evolution, cohort statistics, host genetic background, epitope mapping, clinical, immunological, and epidemiological data will be cross-referenced to viral sequences to allow for subsequent association studies (AU) | |
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