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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of Delivery Method on Neuroprotection by Bone Marrow Mononuclear Cell Therapy following Ventral Root Reimplantation with Fibrin Sealant

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Autor(es):
Barbizan, Roberta [1] ; Castro, Mateus V. [1] ; Barraviera, Benedito [2] ; Ferreira, Jr., Rui S. [2] ; Oliveira, Alexandre L. R. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Dept Struct & Funct Biol, Lab Nerve Regenerat, Campinas, SP - Brazil
[2] Univ Estadual Paulista, Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 9, n. 8 AUG 26 2014.
Citações Web of Science: 13
Resumo

The present work compared the local injection of mononuclear cells to the spinal cord lateral funiculus with the alternative approach of local delivery with fibrin sealant after ventral root avulsion (VRA) and reimplantation. For that, female adult Lewis rats were divided into the following groups: avulsion only, reimplantation with fibrin sealant; root repair with fibrin sealant associated with mononuclear cells; and repair with fibrin sealant and injected mononuclear cells. Cell therapy resulted in greater survival of spinal motoneurons up to four weeks post-surgery, especially when mononuclear cells were added to the fibrin glue. Injection of mononuclear cells to the lateral funiculus yield similar results to the reimplantation alone. Additionally, mononuclear cells added to the fibrin glue increased neurotrophic factor gene transcript levels in the spinal cord ventral horn. Regarding the motor recovery, evaluated by the functional peroneal index, as well as the paw print pressure, cell treated rats performed equally well as compared to reimplanted only animals, and significantly better than the avulsion only subjects. The results herein demonstrate that mononuclear cells therapy is neuroprotective by increasing levels of brain derived neurotrophic factor (BDNF) and glial derived neurotrophic factor (GDNF). Moreover, the use of fibrin sealant mononuclear cells delivery approach gave the best and more long lasting results. (AU)

Processo FAPESP: 10/00729-2 - Emprego de selante de fibrina combinado com Células Tronco Mononucleares de Medula Óssea no reparo de raízes motoras após a avulsão na interface do SNC/SNP
Beneficiário:Roberta Barbizan Petinari
Linha de fomento: Bolsas no Brasil - Doutorado