HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity

Autor(es)

de Amorim, Marcelo R. ^[1] ; Rinaldo, Daniel ^[2] ; do Amaral, Fabiano P. ^[3] ; Vilegas, Wagner ^[3] ; Magenta, Mara A. G. ^[4] ; Vieira, Jr., Gerardo M. ^[5] ; dos Santos, Lourdes C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP - Brazil

^[2] Univ Estadual Paulista, Dept Quim, BR-17033360 Bauru, SP - Brazil

^[3] Univ Estadual Paulista, BR-11350000 Sao Vicente, SP - Brazil

^[4] Univ Santa Cecilia, BR-11045907 Santos, SP - Brazil

^[5] Univ Fed Mato Grosso, Inst Ciencias Nat Humanas & Sociais, BR-78557267 Sinop, MT - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Química Nova; v. 37, n. 7, p. 1122-U227, 2014.
• Citações Web of Science: 4

Resumo

This article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid). (AU)

• Processo FAPESP: 10/10676-3 - ESTUDO QUÍMICO de Tonina fluviatilis Aubl. (Eriocaulaceae)
• Beneficiário: Marcelo Rodrigues de Amorim
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/03882-1 - Estudo de extratos polares de espécies de Eriocaulaceae por técnicas acopladas
• Beneficiário: Lourdes Campaner dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular