Morphological and morphometric analyses of crushed sciatic nerves after application of a purified protein from natural latex and hyaluronic acid hydrogel

Autor(es)

Pereira Barreiros, Vanessa Cristina ^[1] ; Dias, Fernando Jose ^{[2, 3]} ; Iyomasa, Mamie Mizusaki ^[1] ; Coutinho-Netto, Joaquim ^[3] ; de Sousa, Luiz Gustavo ^[2] ; Sassoli Fazan, Valeria Paula ^[3] ; Antunes, Ricardo de Souza ^[1] ; Watanabe, Ii-sei ^[2] ; Mardegan Issa, Joao Paulo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GROWTH FACTORS; v. 32, n. 5, p. 164-170, OCT 2014.
• Citações Web of Science: 9

Resumo

Hyaluronic acid hydrogels (HAHs) have been used as a carrier of substances and factors in the repair of nervous tissue. Natural latex protein (Hevea brasiliensis, F1) has shown positive effects in treating various types of tissues, including peripheral nerves. This study evaluated the F1 associated with a HAH in a controlled crush injury (axonotmesis) of the sciatic nerve in Wistar rats. The samples were photomicrographed for morphometric and quantitative analyzes using ImageJ 1.47k software (NIH, Bethesda, MD). Morphological, quantitative (myelin area/nerve area ratio and capillary density) and morphometric (minimum nerve fiber diameter, G-Ratio) data revealed an improvement in the recovery of the sciatic nerve with the application of HAH and the combination of HAH and F1 after 4 and 8 weeks of nerve injury. The most efficacious results were observed with the combination of both substances, F1 and HAH, revealing the regenerative capacity of this new biomaterial, which was hardly tested on nerve tissue. (AU)

• Processo FAPESP: 11/01983-2 - Avaliação morfológica e morfométrica do nervo isquiático após esmagamento e aplicação da proteína purificada do látex natural (Hevea Brasiliensis) e do hidrogel de ácido hialurônico
• Beneficiário: Vanessa Cristina Pereira Barreiros
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica