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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Morphological and morphometric analyses of crushed sciatic nerves after application of a purified protein from natural latex and hyaluronic acid hydrogel

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Author(s):
Pereira Barreiros, Vanessa Cristina [1] ; Dias, Fernando Jose [2, 3] ; Iyomasa, Mamie Mizusaki [1] ; Coutinho-Netto, Joaquim [3] ; de Sousa, Luiz Gustavo [2] ; Sassoli Fazan, Valeria Paula [3] ; Antunes, Ricardo de Souza [1] ; Watanabe, Ii-sei [2] ; Mardegan Issa, Joao Paulo [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: GROWTH FACTORS; v. 32, n. 5, p. 164-170, OCT 2014.
Web of Science Citations: 9
Abstract

Hyaluronic acid hydrogels (HAHs) have been used as a carrier of substances and factors in the repair of nervous tissue. Natural latex protein (Hevea brasiliensis, F1) has shown positive effects in treating various types of tissues, including peripheral nerves. This study evaluated the F1 associated with a HAH in a controlled crush injury (axonotmesis) of the sciatic nerve in Wistar rats. The samples were photomicrographed for morphometric and quantitative analyzes using ImageJ 1.47k software (NIH, Bethesda, MD). Morphological, quantitative (myelin area/nerve area ratio and capillary density) and morphometric (minimum nerve fiber diameter, G-Ratio) data revealed an improvement in the recovery of the sciatic nerve with the application of HAH and the combination of HAH and F1 after 4 and 8 weeks of nerve injury. The most efficacious results were observed with the combination of both substances, F1 and HAH, revealing the regenerative capacity of this new biomaterial, which was hardly tested on nerve tissue. (AU)

FAPESP's process: 11/01983-2 - Morphological and morphometric analysis of sciatic nerve crushed and application of purified protein from natural latex (Hevea brasiliensis) and hyaluronic acid hydrogel
Grantee:Vanessa Cristina Pereira Barreiros
Support Opportunities: Scholarships in Brazil - Scientific Initiation