Recently an alternative treatment widely used in the repair of peripheral nerve tissue is low-level laser therapy (LLLT), but the physical parameters used are varied between studies. This project aims to evaluate 3 energy densities used in the application of LLLT in the treatment of lesions in peripheral nerves, using the crush injury for rat sciatic nerve (15 kgf, 5.2 MPa, 10 min). It will be used 35 Wistar rats (B, 200-250 g) divided into 5 groups (n = 7): GC - control; GLe - injured nerve without treatment; GL4 - injured nerve and treated with 4 J/cm2 of LLLT; GL10 - injured nerve and treated with 10 J/cm2 LLLT; and GL50 - injured nerve and treated with 50 J/cm2 LLLT. The animals will be sacrificed 2 weeks after the injury by anesthetic overdose (Ketamine / Xylazine - 150/10 mg / kg), the sciatic nerve samples will be obtained. The material obtained will be fixed (4% PFA), included in Leica® historesin, sliced (3 µm), mounted on slides and stained with Sudan black and neutral red method for historesin. After staining the slides will be photomicrographed and them will be performed the morphological, quantitative (myelin density and density of blood capillaries) and morphometric analysis (maximum and minimum diameters of nerve fibers axon diameter and G-ratio of myelin thickness) with the use of the "ImageJ" software and statistical analysis to compare the results among the groups.
News published in Agência FAPESP Newsletter about the scholarship: