Deposition of organic-inorganic hybrid coatings over 316L surgical stainless steel and evaluation on vascular cells

Autor(es)

Doro, F. G. ^{[1, 2]} ; Ramos, A. P. ^[1] ; Schneider, J. F. ^[3] ; Rodrigues-Filho, U. P. ^[4] ; Veiga, M. A. M. S. ^[1] ; Yano, C. L. ^[5] ; Negreti, A. ^[5] ; Krieger, M. H. ^[5] ; Tfouni, E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Bahia, Inst Quim, Dept Quim Geral & Inorgan, BR-40170290 Salvador, BA - Brazil

^[3] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13563120 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13563120 Sao Carlos, SP - Brazil

^[5] Univ Estadual Campinas, UNICAMP, Dept Fisiol & Biofis, BR-13081970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Canadian Journal of Chemistry; v. 92, n. 10, p. 987-995, OCT 2014.
• Citações Web of Science: 4

Resumo

Surface coating of metallic materials using the sol-gel technique is a suitable approach to obtain hybrid materials with improved properties for biomedical applications. In this study, an AISI 316L stainless steel surface was coated with ormosils prepared from tetraethylsiloxane and 3-glycidoxypropyltrimethoxysilane or polydimethylsiloxane. The characterization of structural and surface properties was performed by several techniques. Surface microstructure, morphology, and energy are dependent on organosilane type and content. Chemical stability of coatings was investigated by static immersion tests in phosphate buffer solution at 37 degrees C, and silicon leaching after 21 days was found to be in the range of similar to 200-300 mu g L-1. Mechanical adhesion was found to be within 1.0 and 3.7 N cm(-1). The interaction of the samples and materials in the cardiovascular environment was investigated through cellular behavior. Biological assays were performed with slides to avoid any cytotoxic effects on human endothelial cells (HUVEC) and rabbit arterial smooth muscle cells (RASM). No significant alterations were observed after 24 h in the viability of RASM and HUVEC cells exposed to different coatings. No increase of HUVEC or RASM migration was observed after 24 h as evaluated by transwell migration assay. The hybrid materials showed suitable properties for potential application as biomaterials in cardiovascular environment as well as for incorporation of bioactive species with the aim to prepare drug-eluting stents. (AU)

• Processo FAPESP: 11/07376-0 - Novos materiais eluidores de óxido nítrico com capacidade de prevenir a reestenose e promover a reendotelização vascular: avaliações in vitro
• Beneficiário: Marta Helena Krieger
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/10674-0 - Avaliação da capacidade de prevenção da reestenose e promoção de endotelização por meio da atividade biológica de novos materiais eluidores de óxido nítrico
• Beneficiário: Claudia Lumy Yano
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/53266-4 - Química, fotoquímica e aplicações biológicas de complexos de rutênio com óxido nítrico e assemelhados de solução a materiais
• Beneficiário: Elia Tfouni
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 03/09578-3 - Nitrosilo complexos de rutenio imobilizados em matrizes solidas.
• Beneficiário: Fabio Gorzoni Doro
• Modalidade de apoio: Bolsas no Brasil - Doutorado