Effect of organic loading rate on hydrogen production from sugarcane vinasse in thermophilic acidogenic packed bed reactors

Autor(es)

Nunes Ferraz Junior, Antonio Djalma ^{[1, 2]} ; Wenzel, Jorge ^[3] ; Etchebehere, Claudia ^[3] ; Zaiat, Marcelo ^[2]

Afiliação do(s) autor(es)

^[1] CTBE Brazilian Bioethanol Sci & Technol Lab CNPEM, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Ctr Res Dev & Innovat Environm Engn, Biol Proc Lab, BR-1356312 Sao Carlos, SP - Brazil

^[3] Inst Invest Biol Clemente Estable, Lab Ecol Microbiana, Dept Bioquim & Genom Microbiana, Montevideo - Uruguay

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 39, n. 30, p. 16852-16862, OCT 13 2014.
• Citações Web of Science: 43

Resumo

This study evaluated the effect of organic loading rate (OLR) on hydrogen production in up-flow anaerobic packed bed reactors (APBR) continuously fed with sugarcane vinasse. Four thermophilic up-flow APBR were operated in parallel at different ORL. Continuous hydrogen production was detected. The optimum OLR of 84.2 kg-COD m(-3) d(-1) was assessed by polynomial adjustment, which predicted a maximum Volumetric Hydrogen Production (VHP) and hydrogen yield (Y-H2) of 1117.2 mL-H-2 d(-1) L-reactor(-1) and 2.4 mol-H-2 mol(total carbohydrates)(-1), respectively. The microbial composition was monitored using 16S rRNA gene by Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and quantification of Fe-hydrogenase gene by real-time PCR which was affected by the OLR. The number of the Fe-hydrogenase genes was proportional to the monitored hydrogen production and yield. Hydrogen-producing strains were isolated, and the 16S rRNA gene sequences were highly homologous to those of Thermoanaerobacterium thermosaccarolyticum. The ability of vinasse as substrate for hydrogen production was confirmed for both strains. Copyright (c) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/15984-0 - Produção de bioenergia no tratamento de águas residuárias e adequação ambiental dos efluentes e resíduos gerados
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático