Quantification and characterization of Salmonella spp. isolates in sewage sludge with potential usage in agriculture

Autor(es)

Krzyzanowski, Jr., Flavio ^{[1, 2]} ; Zappelini, Lincohn ^{[1, 2]} ; Martone-Rocha, Solange ^[1] ; Dropa, Milena ^[1] ; Matte, Maria Helena ^[1] ; Nacache, Flavia ^[1] ; Pepe Razzolini, Maria Tereza ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, BR-01246904 Sao Paulo - Brazil

^[2] Inst Fed Educ Ciencia & Tecnol Sao Paulo Brasil, BR-01109010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Microbiology; v. 14, OCT 22 2014.
• Citações Web of Science: 9

Resumo

Background: This study aims to scrutinize Salmonella spp. and its serotypes in sewage sludge samples from wastewater treatment plants, and assesses the presence of virulence genes and antibiotics resistant to the profile. Samples (n = 54) were collected and analyzed in accordance with the EPA Method 1682/2006. For positive serological reaction, 40 strains were selected for PCR analyses and detection of spvC, invA and sseL virulence genes, plasmid presence and resistance to antibiotics. Results: Salmonella spp. was detected in 38.9% of the samples collected (<0.006473 to 12.19 MPN/gTS). The most prevalent serotype was Salmonella Infantis. All Salmonella spp. (n = 35) presented at least one of the three virulence genes mentioned above and 40% harboured plasmids. Salmonella Typhimurium strains were isolated harbouring at least one of the following virulence genes: spvC, invA or sseL. Four Salmonella spp. isolates were resistant to tetracycline; three were resistant to trimethoprim-sulfamethoxazole, and one isolate was resistant to ciprofloxacin. Two Salmonella spp. strains presented multi resistance to antimicrobial agents. Conclusions: The results obtained demonstrated that Salmonella spp. have been found in sewage sludge, thus it is essential to set measures to mitigate human health risks when it is intended to be applied on agricultural soils. (AU)

• Processo FAPESP: 10/05664-6 - Utilização da avaliação quantitativa de risco microbiológico (AQRM) na estimativa da probabilidade de infecção associado à prática de aplicação de biossólidos no solo
• Beneficiário: Maria Tereza Pepe Razzolini
• Modalidade de apoio: Auxílio à Pesquisa - Regular