Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal adenocarcinoma and melanoma cells: An in vitro study

Autor(es)

Martinez, Elizabeth Ferreira ^[1] ; Dias Demasi, Ana Paula ^[1] ; Napimoga, Marcelo Henrique ^[1] ; Barcellos Silva, Carolina Amalia ^[1] ; Navarini, Natalia Festugatto ^[1] ; Araujo, Ney Soares ^[1] ; De Araujo, Vera Cavalcanti ^[1]

Afiliação do(s) autor(es)

^[1] Sao Leopoldo Mand Inst & Res Ctr, Dept Oral Pathol, BR-13045610 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oncology Letters; v. 9, n. 1, p. 313-317, JAN 2015.
• Citações Web of Science: 0

Resumo

Myoepithelial cells have been implicated in the regulation of the transition from in situ to invasive neoplasia in salivary gland tumors. Considering the importance of the microenvironment of the tumor, the present in vitro study therefore analyzed the morphological and phenotypic changes undergone by benign myoepithelial cells from pleomorphic adenoma (PA) stimulated by tumor-conditioned medium. The benign myoepithelial cells were obtained from PA and were cultured with fibronectin extracellular matrix protein, supplemented with tumor-conditioned medium, which was harvested from breast ductal adenocarcinoma AU-565 and melanoma Hs 852.T cells. The morphological alterations were assessed by immunofluorescence analysis using vimentin antibody. The -smooth muscle actin (-SMA) and fibroblast growth factor (FGF)-2 proteins were analyzed by indirect immunofluorescence and quantitative polymerase chain reaction (qPCR). No morphological changes were observed in the myoepithelial cells cultured in fibronectin protein under stimulation from either tumor-conditioned medium. The immunofluorescence results, which were supported by qPCR analysis, revealed that only -SMA was upregulated in the fibronectin substratum, with or without tumor-conditioned medium obtained from breast ductal adenocarcinoma and melanoma cells. No significant difference in FGF-2 mRNA expression was detected when the cells were cultured either in the tumor-conditioned medium or in the fibronectin substratum. The tumor-conditioned medium harvested from breast ductal adenocarcinoma and melanoma did not affect myoepithelial cell differentiation and function, which was reflected by the fact that there was no observed increase in -SMA and FGF-2 expression, respectively. (AU)

• Processo FAPESP: 11/14053-3 - Estudo in vitro da expressão de moléculas de adesão em áreas mimetizadas de neoplasia maligna in situ
• Beneficiário: Elizabeth Ferreira Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58721-7 - Alterações morfológicas e fenotípicas das células mioepiteliais estimuladas por células de carcinoma
• Beneficiário: Elizabeth Ferreira Martinez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado