| Texto completo | |
| Autor(es): |
Chiavegatto, Silvana
[1, 2, 3]
;
Sauce, Bruno
[4]
;
Ambar, Guilherme
[2]
;
Cheverud, James M.
[5]
;
Peripato, Andrea C.
[6, 4]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept & Inst Psychiat, Sao Paulo - Brazil
[3] Natl Inst Dev Psychiat Childhood & Adolescence IN, Sao Paulo - Brazil
[4] Univ Fed Sao Carlos, Dept Genet & Evolut, Biol Sci & Hlth Ctr, BR-13565905 Sao Carlos, SP - Brazil
[5] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 - USA
[6] Univ Fed Sao Paulo, Dept Biosci, Santos, SP - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BRAIN AND BEHAVIOR; v. 2, n. 4, p. 365-376, JUL 2012. |
| Citações Web of Science: | 9 |
| Resumo | |
Maternal care is essential in mammals, and variations in the environment provided by mothers may directly influence the viability of newborns and emotional behavior later in life. A previous study investigated genetic variations associated with maternal care in an intercross of LG/J and SM/J inbred mouse strains and identified two single-locus QTLs (quantitative trait loci). Here, we selected three candidate genes located within these QTLs intervals; Oxt on chromosome 2, and FosB and Peg3 on chromosome 7 and tested their association with maternal care. LG/J females showed impaired postpartum nest building and pup retrieval, a one-day delay in milk ejection, reduced exploratory activity, and higher anxiety-like behavior when compared to SM/J females. The nucleotide sequences of Oxt and FosB were similar between strains, as were their hypothalamic expression levels. Conversely, Peg3 nucleotide sequences showed four nonsynonymous replacement substitutions on LG/J dams, T11062G, G13744A, A13808G, and G13813A, and a 30 base pair (10 aa) in tandem repeat in the coding region with three copies in SM/J and five copies in LG/J. Maternal care impaired LG/J mothers express 37% lower Peg3 mRNA levels in the hypothalamus on the second postpartum day. We also found an association of the Peg3 repeat-variant and poor maternal care in F-2 heterozygote females derived from a LG/J x SM/J intercross. These results may suggest that the maternally imprinted Peg3 gene is responsible for the single-locus QTL on chromosome 7 that has been shown to influence maternal care in these strains. Furthermore, these data provide additional support for an epigenetic regulation of maternal behavior. (AU) | |
| Processo FAPESP: | 04/14583-9 - Bases genéticas do cuidado materno: uma visão detalhada |
| Beneficiário: | Andréa Cristina Peripato |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 05/56353-2 - Bases genetica do cuidado materno: uma visao detalhada. |
| Beneficiário: | Andréa Cristina Peripato |
| Modalidade de apoio: | Bolsas no Brasil - Jovens Pesquisadores |
| Processo FAPESP: | 09/01333-8 - Administração prolongada do ácido 13 cis-retinóico (isotretinoína) em camundongos machos adolescentes: comportamentos emocionais e níveis de RNA mensageiro de vários receptores de serotonina centrais |
| Beneficiário: | Silvana Chiavegatto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |