Effects of eccentric exercise in rehabilitation of phasic and tonic muscles after leg immobilization in rats

Autor(es)

Cornachione, Anabelle S. ^[1] ; Cacao-Benedini, Leticia O. ^[1] ; Chesca, Deise Lucia ^[2] ; Martinez, Edson Z. ^[3] ; Mattiello-Sverzut, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biomech Med & Rehabilitat Locomotor Apparatu, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pathol, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Social Med, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA HISTOCHEMICA; v. 116, n. 8, p. 1216-1224, 2014.
• Citações Web of Science: 2

Resumo

Eccentric exercise is an essential resource for skeletal muscle rehabilitation following muscle disuse however, abnormalities linked to the tissue recuperation require further research. Our aim was analyze the adaptation ability of rehabilitated muscular tissue in rats during different periods of eccentric training after 10 days of limb immobilization. Twenty-seven Wistar rats were divided into six groups: immobilized 10 days, immobilized and eccentric trained for 10 days, immobilized and eccentric trained for 21 days, and three age-matched control groups. After sacrifice, soleus and plantaris muscles were frozen, cut and stained for general histology using hematoxylin and eosin and Gomori trichrome methods and immunohistochemical methods for fiber typing (mATPase, NADH2-TR), for capillaries (CD31) and intermediate filaments (desmin, vimentin) and high resolution microscopy of resin embedded material. Immobilization resulted in more intense morphological alterations in soleus muscles such as formation of target fibers, nuclear centralization, a reduction in the number of type I fibers, diameter of type I, IIA, HAD fibers, and capillaries. After 10 days of eccentric training, increases in the nuclear centralization and the number of lobulated fibers were observed. This period was insufficient to reestablish the capillary/fiber (C/F) ratio and distribution of fiber types as that observed in the control group. However, 21 days of rehabilitation allowed the reversal of all morphological and quantitative abnormalities. For the plantaris muscles, 10-days of training restored their basic characteristics. Despite the fact that immobilization affected soleus and plantaris muscles, 10 days of eccentric training was insufficient to restore the morphological characteristics of soleus muscles, which was not the case observed in plantaris muscle. (C) 2014 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 07/52506-4 - Estudos das adaptações musculares geradas pelos protocolos de alongamento e exercício excêntrico aplicados após imobilização do tornozelo de ratas em desenvolvimento pós-natal e ratas adultas
• Beneficiário: Ana Cláudia Mattiello-Sverzut
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/52961-3 - Efeitos da aplicação de diferentes protocolos de reabilitação em músculos esqueléticos de ratas submetidas previamente a imobilização do membro posterior direito
• Beneficiário: Beatriz Armelin de Almeida Accordi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 07/51715-9 - Efeitos da aplicação de diferentes protocolos de reabilitação em músculos esqueléticos de ratas submetidas previamente a imobilização do membro posterior direito
• Beneficiário: Anabelle Silva Cornachione
• Modalidade de apoio: Bolsas no Brasil - Doutorado